大肠杆菌实验室菌株的生长和维持。
Growth and Maintenance of Escherichia coli Laboratory Strains.
机构信息
Plymouth State University, Plymouth, New Hampshire.
出版信息
Curr Protoc. 2021 Jan;1(1):e20. doi: 10.1002/cpz1.20.
Abstract
Escherichia coli is a Gram-negative bacterium, commonly used in both teaching and research laboratories. This article includes protocols for the growth and maintenance of E. coli in any teaching- or research-associated laboratory. © 2021 Wiley Periodicals LLC. Basic Protocol 1: Growth of E. coli from frozen stocks Basic Protocol 2: Growth of E. coli in liquid media Basic Protocol 3: Enumeration of E. coli on solid media Basic Protocol 4: Storage of E. coli frozen stocks in glycerol Basic Protocol 5: Storage of E. coli in agar stabs Basic Protocol 6: Growth curve of E. coli liquid culture.
摘要
大肠杆菌是一种革兰氏阴性细菌，常用于教学和研究实验室。本文包含在任何教学或研究相关实验室中培养和维持大肠杆菌的方案。© 2021 Wiley Periodicals LLC。基本方案 1：从冷冻库存中生长大肠杆菌基本方案 2：在液体培养基中生长大肠杆菌基本方案 3：在固体培养基上计数大肠杆菌基本方案 4：用甘油储存大肠杆菌冷冻库存基本方案 5：用琼脂斜面试管储存大肠杆菌基本方案 6：大肠杆菌液体培养的生长曲线。
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