Ormsbee R, Peacock M, Philip R, Casper E, Plorde J, Gabre-Kidan T, Wright L
Am J Epidemiol. 1977 Mar;105(3):261-71. doi: 10.1093/oxfordjournals.aje.a112382.
Microagglutination (MA), microcomplement-fixation (CF), indirect microimmunofluorescence (micro-IF) and Weil-Felix (WF) tests were compared for sensitivity and specificity in detection of antibodies against Rickettsia prowazekii and for serologic diagnosis of epidemic typhus fever. Paired sera from hospitalized Ethiopian patients suffering from febrile illness were examined. Purified particulate rickettsial antigens used in MA, CF and micro-IF tests were made from infected yolk sacs by differential centrifugation from molar NaCl and from NaCl-sucrose, D20=1.1491, pH 5.5. In sensitivity the tests ranked micro-IF greater than MA greater than CF greater than WF. Use of specific anti-IgG and anti-IgM sera in parallel micro-IF tests made it possible to differentiate cases of recrudescent epidemic typhus (Brill-Zinsser disease) from primary epidemic typhus cases. Antibodies reacting in high titer with R. canada were produced by 86% of patients with rising antibody titers against R. prowazekii.
对微量凝集试验（MA）、微量补体结合试验（CF）、间接微量免疫荧光试验（微量IF）和外斐试验（WF）在检测普氏立克次体抗体及流行性斑疹伤寒血清学诊断中的敏感性和特异性进行了比较。检测了埃塞俄比亚因发热性疾病住院患者的配对血清。MA、CF和微量IF试验中使用的纯化颗粒性立克次体抗原是通过从感染的卵黄囊中用摩尔NaCl和NaCl-蔗糖（D20 = 1.1491，pH 5.5）进行差速离心制备的。在敏感性方面，这些试验的排序为：微量IF＞MA＞CF＞WF。在平行微量IF试验中使用特异性抗IgG和抗IgM血清能够区分复发型流行性斑疹伤寒（布里尔-津瑟病）病例和原发性流行性斑疹伤寒病例。86%抗普氏立克次体抗体滴度升高的患者产生了与加拿大立克次体高滴度反应的抗体。
