Xu Liai, Liu Xi, Pan Xiangdong, Xu Sinan, Wu Qinglian, Ma Chengyi, Lei Zupei, Ying Yeqing
Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable, Ministry of Agriculture and Rural Affairs, Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A&F University, Hangzhou, Zhejiang, China.
Wuyanling National Nature Reserve Management Center of Zhejiang, Wenzhou, Zhejiang, China.
Front Plant Sci. 2025 May 1;16:1537273. doi: 10.3389/fpls.2025.1537273. eCollection 2025.
, a medicinal plant in Chinese herbal medicine, lacks comprehensive metabolite data to explain its health benefits despite its long-standing use. Here, widely targeted metabolome at six different tissues of was investigated, identifying 2,140 metabolites, including flavonoids, phenolic acids, amino acids, lipids, and alkaloids. Among them, 305 were annotated as key active ingredients, and 364 were active pharmaceutical ingredients for nine human disease-resistance, with 206 co-annotated. Metabolic profiles varied significantly across tissues, with medicinally active metabolites highly concentrated in lateral roots and inflorescences, indicating great medical potential. Notably, the lateral root, rather than the main root, was the primary source of root-derived bioactive metabolites. Additionally, KEGG analysis demonstrated that secondary metabolic pathways, especially "isoflavonoid biosynthesis" and "flavonoid biosynthesis" pathways, played important roles. Overall, lateral roots and inflorescences exhibit the strongest potential for disease treatment, particularly for chronic and multifactorial diseases. This study significantly advances our understanding of 's chemical composition and underscores its potential as a valuable resource for novel therapeutic applications, providing a strong foundation for further investigation into its pharmacological properties and drug development prospects.
作为一种在中草药中使用的药用植物，尽管其长期被使用，但缺乏全面的代谢物数据来解释其健康益处。在此，对该植物六个不同组织的广泛靶向代谢组进行了研究，鉴定出2140种代谢物，包括黄酮类、酚酸类、氨基酸类、脂类和生物碱类。其中，305种被注释为关键活性成分，364种是针对九种人类抗病性的活性药物成分，有206种是共同注释的。不同组织间的代谢谱差异显著，药用活性代谢物高度集中在侧根和花序中，表明其具有巨大的药用潜力。值得注意的是，侧根而非主根是根源性生物活性代谢物的主要来源。此外，KEGG分析表明次生代谢途径，特别是“异黄酮生物合成”和“黄酮生物合成”途径发挥了重要作用。总体而言，侧根和花序在疾病治疗方面表现出最强的潜力，尤其是对慢性和多因素疾病。这项研究显著推进了我们对该植物化学成分的理解，并强调了其作为新型治疗应用宝贵资源的潜力，为进一步研究其药理特性和药物开发前景奠定了坚实基础。
