Sharma Shweta, Jain Suman, Chittora Reena, Chaudhry Rama, Nag Tapas Chandra, A Elavarasi, Radhakrishnan Divya M, Pandey Shivam, Das Suman, Bamola V Deepak, Kochhar Kanwal Preet
Department of Physiology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
Department of Microbiology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
Ann Neurosci. 2025 May 13:09727531251335746. doi: 10.1177/09727531251335746.
Parkinson's disease (PD) is a neurodegenerative disorder that affects both motor and non-motor functions of the body. Recently, scientists have attributed that gut dysbiosis plays a crucial role in the development and progression of PD.
In this study, we aim to determine the role of probiotic supplementation on gut dysbiosis, inflammatory responses at the systemic level, neurodegeneration and motor deficits.
To develop a rat model of PD, 6-hydroxydopamine was bilaterally injected into the striatum. Starting from the first week after surgery, probiotics were administered orally for a period of four weeks. In this study, 18 rats were randomly divided into three groups ( = 6 each): the sham group, the PD group and the PD + Probiotics group. Motor function was measured using paw print analysis, while non-motor function was assessed through gastric emptying. Neuronal survival was evaluated with cresyl violet staining, and blood-based biomarkers were measured to assess inflammation. General body conditions, including body weight, food intake and water intake, were monitored daily.
Probiotic supplementation significantly improved body weight, food intake, water intake and gastric emptying, along with improving gait. Additionally, probiotic supplementation reduced neuronal loss in the brains of PD rats. There was also a reduction in inflammatory markers, such as interleukin-6 and glutaminase, along with an elevation in brain-derived neurotrophic factor levels in the serum. This study is the first to demonstrate the beneficial effects of probiotics in improving motor deficits and gastric emptying in a PD model.
Our findings suggest that probiotic supplementation has the potential to slow down PD progression by preventing gut dysbiosis and neurodegeneration.
帕金森病（PD）是一种影响身体运动和非运动功能的神经退行性疾病。最近，科学家们认为肠道微生物群失调在PD的发生和发展中起着关键作用。
在本研究中，我们旨在确定补充益生菌对肠道微生物群失调、全身炎症反应、神经退行性变和运动功能障碍的作用。
为建立PD大鼠模型，将6-羟基多巴胺双侧注射到纹状体中。从手术后第一周开始，口服给予益生菌，持续四周。在本研究中，18只大鼠被随机分为三组（每组n = 6）：假手术组、PD组和PD + 益生菌组。使用爪印分析测量运动功能，通过胃排空评估非运动功能。用甲酚紫染色评估神经元存活情况，并测量血液中的生物标志物以评估炎症。每天监测一般身体状况，包括体重、食物摄入量和饮水量。
补充益生菌显著改善了体重、食物摄入量、饮水量和胃排空，同时改善了步态。此外，补充益生菌减少了PD大鼠大脑中的神经元损失。炎症标志物如白细胞介素-6和谷氨酰胺酶也有所减少，同时血清中脑源性神经营养因子水平升高。本研究首次证明了益生菌在改善PD模型中的运动功能障碍和胃排空方面的有益作用。
我们的研究结果表明，补充益生菌有可能通过预防肠道微生物群失调和神经退行性变来减缓PD的进展。
