Bose Samrat, Nag Tapas Chandra, Dey Soumil, Sundd Monica, Jain Suman
Department of Physiology, All India Institute of Medical Sciences, New Delhi, Delhi, India.
Department of Biomedical Sciences, Marquette University, Milwaukee, Wisconsin, USA.
Ann Neurosci. 2023 Jan;30(1):11-19. doi: 10.1177/09727531221117634. Epub 2022 Aug 27.
Parkinson's disease (PD) is a progressive neurodegenerative disorder that mainly affects the aged population. Transcranial magnetic field (MF) stimulation has shown to provide temporary motor recovery in neurological disorders.
The aim of this study was to understand the cellular and molecular mechanism of low-intensity MF stimulation (17.96 µT; 50Hz; 2 h/day, four weeks) in a rat model of severe PD.
A clinically relevant, bilateral striatal 6-hydroxydopamine (6-OHDA) lesioned rat model of severe PD was employed to test the efficacy of low-intensity MF stimulation in the management of motor symptoms. The mechanism of action of MF was dissected by assessing the microglial activation, tissue ultrastructure, and cerebrospinal fluid (CSF) metabolomics using microdialysis.
We observed a significant improvement in the postural balance and gait after MF exposure with a significant reduction in the number of activated microglia. There was an improvement in striatal dopaminergic innervation and glutamate levels but it did not reach a level of statistical significance.
MF stimulation helped ameliorate the motor deficits and reduced inflammation but was unable to provide a significant change in terms of dopaminergic innervation and metabolic profile in the severe 6-OHDA PD rat model.
帕金森病（PD）是一种主要影响老年人群的进行性神经退行性疾病。经颅磁场（MF）刺激已显示可在神经系统疾病中提供暂时的运动恢复。
本研究的目的是了解低强度MF刺激（17.96微特斯拉；50赫兹；每天2小时，共四周）在重度PD大鼠模型中的细胞和分子机制。
采用临床上相关的双侧纹状体6-羟基多巴胺（6-OHDA）损伤的重度PD大鼠模型，以测试低强度MF刺激在管理运动症状方面的疗效。通过使用微透析评估小胶质细胞活化、组织超微结构和脑脊液（CSF）代谢组学来剖析MF的作用机制。
我们观察到MF暴露后姿势平衡和步态有显著改善，活化小胶质细胞数量显著减少。纹状体多巴胺能神经支配和谷氨酸水平有所改善，但未达到统计学显著水平。
在重度6-OHDA PD大鼠模型中，MF刺激有助于改善运动缺陷并减轻炎症，但在多巴胺能神经支配和代谢谱方面未能产生显著变化。
