Mingcheng Wang, Daoqi Liu, Huili Xia, Gailing Wang, Chaoying Liu, Yanan Guo, Aizhen Guo
Country National Laboratory of Agricultural Microbiology, Wuhan, Hubei, China.
Country College of Veterinary Medicine, Wuhan, Hubei, China.
Front Microbiol. 2025 May 1;15:1437056. doi: 10.3389/fmicb.2024.1437056. eCollection 2024.
Ammonia is the primary component of malodorous substances in chicken farms. Currently, the microbial ammonia reduction is considered a potential method due to its low cost, high safety, and environmental friendliness. sp. Z392 can significantly reduce the ammonia level in broiler coops. However, the mechanisms of ammonia nitrogen reduction by sp. Z392 remain unclear. To explore the mechanisms of ammonia reduction by sp. Z392, the transcriptome and metabolome analysis of sp. Z392 under high ammonium sulfate level were conducted. It was found that the transcription levels of genes related to purine metabolism (, , , , ) and arginine metabolism (, , , , ) were decreased under high ammonium sulfate environment, and the levels of intermediate products such as ornithine, arginine, IMP, and GMP also were also decreased. In addition, the gene in nitrogen metabolism was upregulated, and intracellular nitrite content increased by 2.27 times than that without ammonium sulfate. These results suggested that under high ammonium sulfate level, the flux of purine and arginine metabolism pathways in sp. Z392 might decrease, while the flux of nitrogen metabolism pathway might increase, resulting in increased nitrite content and NH release. To further verify the effect of the gene on ammonia removal, was successfully overexpressed and knocked out in sp. Z392. Overexpression exhibited the most ammonia reduction capability, the ammonia concentration of overexpression group decreased by 43.33% than that of without sp. group, and decreased by 14.17% than that of sp. Z392 group. In conclusion, sp. Z392 might reduce the release of NH by reducing the flux of purine and arginine metabolisms, while enhancing ammonia assimilation to form nitrite. In this context, might be one of the key genes to reduce ammonia.
氨气是养鸡场恶臭物质的主要成分。目前，微生物降氨因其成本低、安全性高和环境友好性而被视为一种潜在方法。Z392菌株能显著降低肉鸡舍内的氨气水平。然而，Z392菌株降氨氮的机制仍不清楚。为探究Z392菌株降氨的机制，对高硫酸铵水平下的Z392菌株进行了转录组和代谢组分析。结果发现，在高硫酸铵环境下，与嘌呤代谢（相关基因）和精氨酸代谢（相关基因）的转录水平降低，鸟氨酸、精氨酸、肌苷酸和鸟苷酸等中间产物水平也降低。此外，氮代谢中的基因上调，细胞内亚硝酸盐含量比无硫酸铵时增加了2.27倍。这些结果表明，在高硫酸铵水平下，Z392菌株中嘌呤和精氨酸代谢途径的通量可能降低，而氮代谢途径的通量可能增加，导致亚硝酸盐含量增加和氨释放。为进一步验证该基因对氨去除的作用，在Z392菌株中成功进行了该基因的过表达和敲除。过表达表现出最强的降氨能力，过表达组的氨浓度比无该菌株组降低了43.33%，比Z392菌株组降低了14.17%。总之，Z392菌株可能通过降低嘌呤和精氨酸代谢通量，同时增强氨同化形成亚硝酸盐来减少氨的释放。在此背景下，该基因可能是降氨的关键基因之一。
