MS(4) Life Laboratory of Mass Spectrometry, Health Sciences Postgraduate Program, São Francisco University, Bragança Paulista, São Paulo, CEP 12916-900, Brazil; Graduate Program in Pure and Applied Chemistry (POSQUIPA) Federal University of Western Bahia, Rua Bertioga, 892, Morada Nobre II, Barreiras, Bahia, CEP 47810-059, Brazil.
MS(4) Life Laboratory of Mass Spectrometry, Health Sciences Postgraduate Program, São Francisco University, Bragança Paulista, São Paulo, CEP 12916-900, Brazil.
J Chromatogr B Analyt Technol Biomed Life Sci. 2022 Jul 15;1204:123312. doi: 10.1016/j.jchromb.2022.123312. Epub 2022 May 28.
The Caenorhabditis elegans (C. elegans) is a model organism that has been increasingly used in health and environmental toxicity assessments. The quantification of such elements in vivo can assist in studies that seek to relate the exposure concentration to possible biological effects. Therefore, this study is the first to propose a method of quantitative analysis of 21 ions by ion chromatography (IC), which can be applied in different toxicity studies in C. elegans. The developed method was validated for 12 anionic species (fluoride, acetate, chloride, nitrite, bromide, nitrate, sulfate, oxalate, molybdate, dichromate, phosphate, and perchlorate), and 9 cationic species (lithium, sodium, ammonium, thallium, potassium, magnesium, manganese, calcium, and barium). The method did not present the presence of interfering species, with R varying between 0.9991 and 0.9999, with a linear range from 1 to 100 µg L. Limits of detection (LOD) and limits of quantification (LOQ) values ranged from 0.2319 μg L to 1.7160 μg L and 0.7028 μg L to 5.1999 μg L, respectively. The intraday and interday precision tests showed an Relative Standard Deviation (RSD) below 10.0 % and recovery ranging from 71.0 % to 118.0 % with a maximum RSD of 5.5 %. The method was applied to real samples of C. elegans treated with 200 uM of thallium acetate solution, determining the uptake and bioaccumulated Tl content during acute exposure.
秀丽隐杆线虫（C. elegans）是一种模式生物，越来越多地用于健康和环境毒性评估。体内这些元素的定量分析可以帮助研究将暴露浓度与可能的生物效应联系起来。因此，本研究首次提出了一种通过离子色谱（IC）定量分析 21 种离子的方法，该方法可应用于秀丽隐杆线虫的不同毒性研究。所开发的方法针对 12 种阴离子（氟化物、乙酸盐、氯、亚硝酸盐、溴化物、硝酸盐、硫酸盐、草酸盐、钼酸盐、重铬酸盐、磷酸盐和高氯酸盐）和 9 种阳离子（锂、钠、铵、铊、钾、镁、锰、钙和钡）进行了验证。该方法未发现存在干扰物质，R 值在 0.9991 到 0.9999 之间，线性范围为 1 到 100μg L。检出限（LOD）和定量限（LOQ）值范围分别为 0.2319μg L 到 1.7160μg L 和 0.7028μg L 到 5.1999μg L。日内和日间精密度测试的相对标准偏差（RSD）低于 10.0%，回收率范围为 71.0%到 118.0%，最大 RSD 为 5.5%。该方法应用于用 200μM 醋酸铊溶液处理的秀丽隐杆线虫的实际样品中，测定急性暴露期间的Tl 摄取和生物累积量。
