IV型日本脑炎病毒基因内嵌合株在小鼠体内的体外生长及致病性评估

Evaluation of in vitro growth and pathogenicity in mice of intra-genotype chimeric strains of genotype IV Japanese encephalitis virus.

作者信息

Tajima Shigeru, Faizah Astri Nur, Kobayashi Daisuke, Mulyatno Kris Cahyo, Subekti Sri, Lusida Maria Inge, Mori Yasuko, Isawa Haruhiko, Sawabe Kyoko, Lim Chang-Kweng

机构信息

Department of Virology I, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo, 162-8640, Japan.

Department of Medical Entomology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo, 162-8640, Japan.

出版信息

Arch Virol. 2025 May 16;170(6):130. doi: 10.1007/s00705-025-06317-2.

DOI:10.1007/s00705-025-06317-2

PMID:40377734

Abstract

Two recombinant intra-genotype chimeric genotype IV Japanese encephalitis virus (GIV JEV) strains with the 5' non-coding and C-prM-E regions (5NCME) of the Vietnamese strain VN113 or the Australian strain sw-22-00722-11.Qld (sw-22) were produced using the Indonesian strain 19CxBa-83-Cv as a backbone. The three strains exhibited similar plaque morphology and growth rate in Vero cells. The virus with sw-22 5NCME was less neuroinvasive than 19CxBa-83-Cv, but the virulence of the virus with VN113 5NCME was similar to that of 19CxBa-83-Cv. Thus, our results suggest that the pathogenicity of sw-22 might be lower than that of the other two GIV JEV isolates.

摘要

使用印度尼西亚毒株19CxBa - 83 - Cv作为主干，构建了两种重组的基因内基因型嵌合4型日本脑炎病毒（GIV JEV）毒株，其5'非编码区和C - prM - E区（5NCME）分别来自越南毒株VN113或澳大利亚毒株sw - 22 - 00722 - 11.Qld（sw - 22）。这三种毒株在Vero细胞中表现出相似的蚀斑形态和生长速率。具有sw - 22 5NCME的病毒神经侵袭性低于19CxBa - 83 - Cv，但具有VN113 5NCME的病毒毒力与19CxBa - 83 - Cv相似。因此，我们的结果表明，sw - 22的致病性可能低于其他两种GIV JEV分离株。