Tajima Shigeru, Faizah Astri Nur, Kobayashi Daisuke, Mulyatno Kris Cahyo, Subekti Sri, Lusida Maria Inge, Mori Yasuko, Isawa Haruhiko, Sawabe Kyoko, Lim Chang-Kweng
Department of Virology I, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo, 162-8640, Japan.
Department of Medical Entomology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo, 162-8640, Japan.
Arch Virol. 2025 May 16;170(6):130. doi: 10.1007/s00705-025-06317-2.
Two recombinant intra-genotype chimeric genotype IV Japanese encephalitis virus (GIV JEV) strains with the 5' non-coding and C-prM-E regions (5NCME) of the Vietnamese strain VN113 or the Australian strain sw-22-00722-11.Qld (sw-22) were produced using the Indonesian strain 19CxBa-83-Cv as a backbone. The three strains exhibited similar plaque morphology and growth rate in Vero cells. The virus with sw-22 5NCME was less neuroinvasive than 19CxBa-83-Cv, but the virulence of the virus with VN113 5NCME was similar to that of 19CxBa-83-Cv. Thus, our results suggest that the pathogenicity of sw-22 might be lower than that of the other two GIV JEV isolates.
使用印度尼西亚毒株19CxBa - 83 - Cv作为主干，构建了两种重组的基因内基因型嵌合4型日本脑炎病毒（GIV JEV）毒株，其5'非编码区和C - prM - E区（5NCME）分别来自越南毒株VN113或澳大利亚毒株sw - 22 - 00722 - 11.Qld（sw - 22）。这三种毒株在Vero细胞中表现出相似的蚀斑形态和生长速率。具有sw - 22 5NCME的病毒神经侵袭性低于19CxBa - 83 - Cv，但具有VN113 5NCME的病毒毒力与19CxBa - 83 - Cv相似。因此，我们的结果表明，sw - 22的致病性可能低于其他两种GIV JEV分离株。
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