Yu Xiaohong, Zhang Ya, Wang Xianhao, Luo Yutong, Shao Shuai, Qiu Zhongyang
School of Marine and Biology Engineering, Yancheng Institute of Technology, Yancheng, Jiangsu, China.
Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Huaiyin Normal University, Huaian, Jiangsu, China.
Appl Biochem Biotechnol. 2025 May 16. doi: 10.1007/s12010-025-05281-6.
Organic acid pretreatment is one of the most promising methods to pretreat lignocellulose biomass due to its mild condition and weak corrosion to reactor. In this study, five different organic acids were used for wheat bran pretreatment, and the enzymatic hydrolysis yields of pretreated feedstocks were evaluated. Among these organic acids, oxalic acid pretreatment demonstrated the highest glucose yield of 87.93%. Subsequently, the optimization of oxalic acid pretreatment parameters was conducted including temperature, time, solid-liquid ratio, and acid usage. A high glucose yield of 96.92% was obtained at the optimal conditions: 0.8% of oxalic acid usage, 1:10 of solid-liquid ratio, and 130 ℃ for 15 min. FTIR, XRD and SEM revealed the mechanism of improved hydrolysis efficiency after oxalic acid pretreatment. Finally, the pretreated wheat bran was used for separate hydrolysis and ethanol fermentation (SHF) at 20% (w/w) solids loading. After 36 h, 23.87 g/L ethanol was produced by Saccharomyces cerevisiae DQ1 with all glucose consumed, with the yield of 0.12 g/g dry virgin wheat bran. This study provided a new insight into wheat bran pretreatment for biofuel production by a mild oxalic acid pretreatment method.
由于有机酸预处理条件温和且对反应器腐蚀性弱，因此它是预处理木质纤维素生物质最具前景的方法之一。在本研究中，使用了五种不同的有机酸对麦麸进行预处理，并评估了预处理原料的酶解产率。在这些有机酸中，草酸预处理的葡萄糖产率最高，为87.93%。随后，对草酸预处理参数进行了优化，包括温度、时间、固液比和酸用量。在最佳条件下：草酸用量0.8%、固液比1:10、130℃处理15分钟，葡萄糖产率高达96.92%。傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）和扫描电子显微镜（SEM）揭示了草酸预处理后水解效率提高的机制。最后，将预处理后的麦麸用于在20%（w/w）固体负荷下的分步水解和乙醇发酵（SHF）。36小时后，酿酒酵母DQ1消耗了所有葡萄糖，产生了23.87 g/L乙醇，产率为0.12 g/g干原始麦麸。本研究通过温和的草酸预处理方法为麦麸预处理用于生物燃料生产提供了新的见解。
