College of Biotechnology and Food Engineering, Changzhou University, Changzhou, Jiangsu 213164, China.
College of Biotechnology and Food Engineering, Changzhou University, Changzhou, Jiangsu 213164, China; Advanced Catalysis and Green Manufacturing Collaborative Innovation Center, Changzhou University, Changzhou 213164, China.
Bioresour Technol. 2024 Mar;395:130338. doi: 10.1016/j.biortech.2024.130338. Epub 2024 Jan 17.
Deep eutectic solvent (DES) is a newly-emerged green solvent for efficient pretreatment of lignocellulosic feedstock. To improve the component fractionation performance of neutral DES, p-toluenesulfonic acid (p-TsOH) was employed as catalyst to form a novel ternary DES with benzyltriethylammonium chloride (TEBAC) and glycerol (Gly) for pretreatment of soybean straw. Under the optimum reaction conditions (TEBAC:Gly = 1:12, 1.6 wt% p-TsOH and reacted at 90 °C for 160 min), the lignin and hemicellulose removal from soybean straw were amounted to 92.0 % and 88.2 %, respectively. The pretreated substrate showed satisfactory enzymatic hydrolysis performance, as the glucose and reducing sugar concentrations reached 37.3 g/L and 42.3 g/L, respectively, after 72 h saccharification under the action of cellulase with a relatively low enzyme loading of 10 FPU/g cellulose.This method provides an efficient and mild route for utilization of agricultural waste and production of platform monosaccharides.
深共晶溶剂（DES）是一种新兴的绿色溶剂，可有效预处理木质纤维素原料。为了提高中性 DES 的组分分级性能，采用对甲苯磺酸（p-TsOH）作为催化剂，与苄基三乙基氯化铵（TEBAC）和甘油（Gly）形成一种新型三元 DES，用于预处理大豆秸秆。在最佳反应条件下（TEBAC：Gly=1：12，1.6wt%p-TsOH，在 90°C 下反应 160min），大豆秸秆中的木质素和半纤维素的去除率分别达到 92.0%和 88.2%。预处理后的底物表现出令人满意的酶解性能，在纤维素酶作用下，72h 糖化后葡萄糖和还原糖浓度分别达到 37.3g/L 和 42.3g/L，酶用量相对较低，为 10FPU/g 纤维素。该方法为利用农业废弃物和生产平台单糖提供了一种高效、温和的途径。
