Hauser Theresa, Lawson Paul A, Bevis Durward L, McLaughlin Richard W
School of Liberal Arts and Sciences, Gateway Technical College, Kenosha, WI 53144, USA.
School of Biological Sciences, University of Oklahoma, Norman, OK 73019, USA.
Int J Syst Evol Microbiol. 2025 May;75(5). doi: 10.1099/ijsem.0.006794.
The present study used whole-genome data to clarify the taxonomic assignment of two closely related species. The genus includes four species with validly published names, and . The type species 002-048 (=IAM 15309=KCTC 13936) was isolated from the soil in Japan, while CL-GR16 (=KCCM 42329=DSM 18252) was isolated from the coastal water off the east coast of Korea. Both strains have similar phenotypic and chemotaxonomic characteristics. Anteiso-C was the major fatty acid, followed by anteiso-C and iso-C. There were similar major menaquinones (MK-7). As determined by whole-genome sequencing, the DNA G+C content of 42.53 and 42.66 mol% was found for and , respectively, and they were 99.86% identical in their 16S rRNA gene sequences (1,421 bp). Genomic comparisons showed the average nucleotide identity was 98.29% and 84.80% for digital DNA-DNA hybridization. These values exceeded the threshold values for bacterial species delineation. Using the Type (Strain) Genome Server, both species were identified as . Phylogenetic analysis based on both 16S rRNA gene sequences and whole-genome sequences showed that they form a separate cluster apart from members of other genera. Based on the combined evidence, Kim . ( 2007;57:1554-1560), Krishnamurthi and Chakrabarti ( 2008;58:2287-2291) is proposed to be a later heterotypic synonym of An . ( 2007;57:51-55).
本研究使用全基因组数据来阐明两个近缘物种的分类归属。该属包括四个已有效发表名称的物种，即 和 。模式种002 - 048（=IAM 15309 = KCTC 13936）从日本土壤中分离得到，而CL - GR16（=KCCM 42329 = DSM 18252）从韩国东海岸的沿海水域分离得到。两种菌株具有相似的表型和化学分类特征。anteiso - C是主要脂肪酸，其次是anteiso - C和iso - C。主要甲基萘醌（MK - 7）相似。通过全基因组测序确定， 和 的DNA G + C含量分别为且 42.53 和 42.66 mol%，它们的16S rRNA基因序列（1421 bp）有99.86%的同一性。基因组比较显示，数字DNA - DNA杂交的平均核苷酸同一性分别为98.29%和84.80%。这些值超过了细菌物种界定的阈值。使用类型（菌株）基因组服务器，两种物种均被鉴定为 。基于16S rRNA基因序列和全基因组序列的系统发育分析表明，它们形成了一个与其他属成员分开的单独聚类。基于综合证据，Kim （2007；57：1554 - 1560）、Krishnamurthi和Chakrabarti（2008；58：2287 - 2291）被提议为An . （2007；57：51 - 55）的晚出异名。
