Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
Biodiversity Center Kasetsart University (BDCKU), Bangkok 10900, Thailand.
Int J Syst Evol Microbiol. 2022 May;72(5). doi: 10.1099/ijsem.0.005356.
A polyphasic approach was used to describe strain RB6PN24, a novel actinobacterium isolated from peat swamp forest soil collected from Rayong province, Thailand. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain belonged to the genus and showed the highest sequence similarities to IFO 15206 (98.7 %) and MMS16-CNU292 (98.5 %). Strain RB6PN24 contained major amounts of -diaminopimelic acid, galactose, mannose and ribose in the whole-cell hydrolysates. MK-9(H) and MK-9(H) were the predominant menaquinones of the micro-organism. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, an unidentified lipid, four unidentified aminolipids and six unidentified phospholipids. Mycolic acids were not present. The major fatty acids were iso-C, iso-C, anteiso-C, iso-C, anteiso-C and C. The draft genome size of strain RB6PN24 was 8.09 Mbp, with 72.1 mol% G+C content and predicted to contain at least 44 biosynthetic gene clusters encoding diverse secondary metabolites. Furthermore, the strain exhibited low average nucleotide identity and digital DNA-DNA hybridization values with MMS16-CNU292 (89.1 %, 42.4 %) and DSM 41654 (79.5 %, 25.5 %). The results of phenotypic, chemotaxonomic, genotypic and phylogenetic analyses revealed that strain RB6PN24 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RB6PN24 (=TBRC 14818=NBRC 115116). In addition, the comparison of the whole genome sequences and phenotypic features suggested that and belong to the same species. Therefore, it is proposed that is reclassified as a later heterotypic synonym of .
采用多相分类法描述了从泰国罗勇省泥炭沼泽森林土壤中分离到的一株新型放线菌 RB6PN24 菌株。基于 16S rRNA 基因序列的系统发育分析表明，该菌株属于 属，与 IFO 15206（98.7%）和 MMS16-CNU292（98.5%）的序列相似性最高。RB6PN24 菌株的全细胞水解物中主要含有 -二氨基庚二酸、半乳糖、甘露糖和核糖。MK-9(H)和 MK-9(H) 是该微生物的主要类异戊二烯醌。极性脂类由双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰肌醇、磷脂酰肌醇甘露糖苷、一种未鉴定的脂类、四种未鉴定的氨基脂类和六种未鉴定的磷脂组成。该菌中不存在分枝菌酸。主要脂肪酸为 iso-C 、 iso-C 、 anteiso-C 、 iso-C 、 anteiso-C 和 C 。RB6PN24 菌株的基因组草图大小为 8.09 Mbp，G+C 含量为 72.1 mol%，预测至少含有 44 个生物合成基因簇，编码多种次级代谢产物。此外，该菌株与 MMS16-CNU292（89.1%，42.4%）和 DSM 41654（79.5%，25.5%）的平均核苷酸同一性和数字 DNA-DNA 杂交值较低。表型、化学分类、基因型和系统发育分析的结果表明，RB6PN24 菌株代表了 属的一个新种，因此建议将其命名为 sp. nov.。其模式菌株为 RB6PN24（=TBRC 14818=NBRC 115116）。此外，全基因组序列和表型特征的比较表明， 和 属于同一物种。因此，建议将 重新分类为 的异名。
