Janković Teodora, Mudrić Jelena, Radojičić Vesna, Pejić Lazar, Ćujić Nikolić Nada, Marković Smilja, Menković Nebojša
Institute for Medicinal Plants Research "Dr Josif Pančić", Tadeuša Košćuška 1, Belgrade 11000, Serbia.
Institute for Medicinal Plants Research "Dr Josif Pančić", Tadeuša Košćuška 1, Belgrade 11000, Serbia.
J Pharm Biomed Anal. 2025 Oct 15;264:116968. doi: 10.1016/j.jpba.2025.116968. Epub 2025 May 13.
The roots and rhizomes of Gentiana lutea L. are officially listed in the European Pharmacopeia and numerous national pharmacopeias and are used mainly to treat gastrointestinal disorders. In contrast, the leaves are traditionally smoked for the treatment of angina pectoris, but scientific support and justification for its traditional use are missing. Therefore, the chemical composition and gentian leaves before and after smoking were investigated in this study. A standardized smoking machine was used to generate cigarette smoke and the chemical constituents were analyzed by high-performance liquid chromatography (HPLC). The analysis showed that a significant proportion (68.77 %) of the xanthone isogentisin was transferred intact from the leaves to the smoke stream, making it the only compound detected in the smoke condensate. Thermogravimetric analysis revealed that isogentisin underwent thermal decomposition within the temperature range of 250-448 °C. These findings provide scientific evidence supporting the medicinal potential of gentian leaves, with isogentisin identified as the active constituent. Furthermore, the thermal stability profile of isogentisin offers valuable insights into its behavior during combustion.
黄龙胆的根及根茎被正式列入《欧洲药典》以及众多国家药典，主要用于治疗胃肠道疾病。相比之下，传统上人们会吸食黄龙胆的叶子来治疗心绞痛，但这种传统用法缺乏科学依据和论证。因此，本研究对黄龙胆叶子在吸食前后的化学成分进行了研究。使用标准化吸烟机产生香烟烟雾，并通过高效液相色谱法（HPLC）分析化学成分。分析表明，相当一部分（68.77%）的氧杂蒽酮异秦皮素完整地从叶子转移到了烟雾流中，使其成为在烟雾冷凝物中检测到的唯一化合物。热重分析表明，异秦皮素在250 - 448°C的温度范围内发生热分解。这些发现为黄龙胆叶子的药用潜力提供了科学证据，异秦皮素被确定为活性成分。此外，异秦皮素的热稳定性概况为其在燃烧过程中的行为提供了有价值的见解。
