Zheng Haoran, Guo Jiayu, Wang Han, Wang Yurou, Zhou Wenxiang, Liu Ke, Ma Qiang, Qi Yanfei
School of Public Health, Jilin University, Changchun, Jilin 130021, China.
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
J Hazard Mater. 2025 Aug 15;494:138612. doi: 10.1016/j.jhazmat.2025.138612. Epub 2025 May 12.
Bacteria are prevalent environmental pollutants. Live bacteria can proliferate and spread under appropriate conditions, presenting higher risks compared to non-viable counterparts. However, detecting live bacteria remains a challenge. Adenosine triphosphate (ATP), an energy currency in organisms, offers a reliable biomarker for the live bacteria sensing. Herein, we developed a zeolitic imidazolate framework-90 (ZIF-90) enhanced ATP sensing platform to detect live bacteria. The nanosystem which based on ZIF-90, encapsulating the DNA decorated magnetic beads (MB@S1) through self-assembly. When the S. aureus aptamers on ZIF-90 bonded to bacteria, the skeleton structure of ZIF-90 was disrupted by ATP leakage from live bacteria, leading to the release of MB@S1. Then, the MB@S1 initiated the hybridization chain reaction (HCR) and they were transduced by CRISPR-Cas12a to amplify the signal twice. The proposed ZIF-90 blocking sensing strategy (ZIF-90 strategy) exhibited remarkable sensitivity with the limit of detection (LOD) down to 0.223 pM for ATP detection, about 500 folds lower than the traditional ATP aptamer blocking sensing strategy (aptamer strategy). Furthermore, we used a smartphone for on-site analysis, realizing the quantification of live S. aureus with the LOD of 2.0 CFU/mL. Therefore, the approach possessed great application potential for public health, environment monitoring, bioanalysis and food safety.
细菌是普遍存在的环境污染物。活细菌在适宜条件下能够增殖和传播，与无活性的细菌相比，其风险更高。然而，检测活细菌仍然是一项挑战。三磷酸腺苷（ATP）作为生物体中的能量货币，为活细菌传感提供了一种可靠的生物标志物。在此，我们开发了一种沸石咪唑酯骨架-90（ZIF-90）增强的ATP传感平台来检测活细菌。该纳米系统基于ZIF-90，通过自组装包裹了DNA修饰的磁珠（MB@S1）。当ZIF-90上的金黄色葡萄球菌适配体与细菌结合时，ZIF-90的骨架结构会因活细菌泄漏的ATP而被破坏，导致MB@S1释放。然后，MB@S1引发杂交链式反应（HCR），并通过CRISPR-Cas12a进行信号转导，从而实现信号的二次放大。所提出的ZIF-90阻断传感策略（ZIF-90策略）表现出显著的灵敏度，ATP检测的检测限（LOD）低至0.223 pM，比传统的ATP适配体阻断传感策略（适配体策略）低约500倍。此外，我们使用智能手机进行现场分析，实现了对活金黄色葡萄球菌的定量检测，LOD为2.0 CFU/mL。因此，该方法在公共卫生、环境监测、生物分析和食品安全方面具有巨大的应用潜力。
