Xu Yiyang, Li Ying, Ye Zixuan, Wang Cheng, Cui Pengfei, Zhou Shuwen, Qiu Lin, Wang Jianhao
School of Pharmacy, Changzhou University, Changzhou, China.
J Biomed Mater Res A. 2025 Jun;113(6):e37937. doi: 10.1002/jbm.a.37937.
Wound bacterial infection deteriorates with antibiotic misuse, boosting bacterial drug resistance, threatening human health. Therefore, combining natural antibacterials with efficient broad-spectrum materials offers new solutions. Artemisia argyi is a kind of medicinal plant distributed in Asia, known for its rich biological active compounds. With the advancement of modern analytical technology, Artemisia argyi has shown excellent antibacterial and anti-inflammatory potential, particularly Artemisia argyi essential oil (AAEO) which is considered to be the most active substance in Artemisia argyi. However, its practical application is limited due to its poor water solubility, strong volatility, high sensitivity to light and heat, and its irritating odor. Zeolitic imidazolate framework-8 (ZIF-8) is a type of metal-organic framework material (MOF) with good biocompatibility, simple synthesis, high porosity, and antimicrobial activity. ZIF-8 can be used as an ideal vehicle for the preparation of antibacterial materials. In this study, we loaded AAEO onto ZIF-8 to successfully develop AAEO@ZIF-8 antibacterial nanomaterial. The bactericidal ability and antibacterial mechanism of AAEO@ZIF-8 were evaluated through in vitro experiments such as bacterial coating, Live/Dead staining, and crystal violet staining. It was confirmed that AAEO@ZIF-8 had significant antibacterial activity against both Staphylococcus aureus and Escherichia coli. In in vivo experiments, the wound model using C57BL/6 male mice was established, and wound tissue staining with Gram, CD31 immunohistochemistry, H&E, and Masson staining was performed to evaluate the antibacterial activity of AAEO@ZIF-8. The results showed that AAEO@ZIF-8 exhibited the best wound repair performance in the model. These findings suggested that AAEO@ZIF-8 is a novel and effective antimicrobial nanomaterial targeting pathogenic bacteria. This study successfully transformed the storage mode of AAEO from liquid to solid, providing a promising strategy for the application of AAEO in the treatment of wound bacterial infections.
伤口细菌感染会因抗生素的滥用而恶化，增强细菌耐药性，威胁人类健康。因此，将天然抗菌剂与高效广谱材料相结合提供了新的解决方案。艾草是一种分布于亚洲的药用植物，以其丰富的生物活性化合物而闻名。随着现代分析技术的进步，艾草已显示出优异的抗菌和抗炎潜力，尤其是艾草精油（AAEO），它被认为是艾草中最具活性的物质。然而，由于其水溶性差、挥发性强、对光和热敏感以及有刺激性气味，其实际应用受到限制。沸石咪唑酯骨架材料-8（ZIF-8）是一种金属有机骨架材料（MOF），具有良好的生物相容性、合成简单、高孔隙率和抗菌活性。ZIF-8可作为制备抗菌材料的理想载体。在本研究中，我们将AAEO负载到ZIF-8上，成功开发出AAEO@ZIF-8抗菌纳米材料。通过细菌包被、活/死染色和结晶紫染色等体外实验评估了AAEO@ZIF-8的杀菌能力和抗菌机制。证实AAEO@ZIF-8对金黄色葡萄球菌和大肠杆菌均具有显著的抗菌活性。在体内实验中，建立了使用C57BL/6雄性小鼠的伤口模型，并进行革兰氏染色、CD31免疫组织化学、苏木精-伊红染色和马松染色对伤口组织进行染色，以评估AAEO@ZIF-8的抗菌活性。结果表明，AAEO@ZIF-8在该模型中表现出最佳的伤口修复性能。这些发现表明，AAEO@ZIF-8是一种针对病原菌的新型有效抗菌纳米材料。本研究成功地将AAEO的储存方式从液体转变为固体，为AAEO在伤口细菌感染治疗中的应用提供了一种有前景的策略。
