Ding Yangcheng, Dong Shuangjing, Ding Danna, Chen Xiaoming, Xu Fangxi, Niu He, Xu Jixiao, Fan Yuhang, Chen Ruya, Xia Yijing, Qiu Xiawen, Feng Huajun
Zhejiang Key Laboratory of Ecological Environmental Damage Control and Value Transformation, College of Environmental and Resources Sciences, Zhejiang Agriculture and Forestry University, Hangzhou 311300, PR China.
International Science and Technology Cooperation Platform for Low-Carbon Recycling of Waste and Green Development, School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310018, PR China.
J Hazard Mater. 2025 Aug 15;494:138604. doi: 10.1016/j.jhazmat.2025.138604. Epub 2025 May 12.
Residual antibiotics in aquaculture ecosystems can exert selective pressures on bacterial communities, driving bacteria to acquire antibiotic resistance genes (ARGs) through gene mutations or horizontal gene transfer (HGT). This study investigated the antibiotic resistance risk in freshwater aquaculture ecosystems of Micropterus salmoides in Zhejiang Province. The results revealed that oxytetracycline, ciprofloxacin and florfenicol were up to 300 ng/L, and the proportion of multidrug-resistant genes varied from 32.20 % to 50.70 % in the surveyed aquaculture water. Additionally, approximately 9.80 % of all annotated ARGs were identified as possessing plasmid-mediated horizontal transfer risks. The ARGs host prediction revealed that Actinobacteria carried the highest abundance of ARGs, up to 159.38 (coverage, ×/Gb). Furthermore, the abundance of Paer_emrE, ksgA, ompR and golS were positively correlated with Chlorophyll a concentration (p < 0.05), suggesting that algal blooms might facilitate the evolution and transfer of ARGs. Correlations between ARG abundances and total phosphorus, total nitrogen, pH, electrical conductivity indicated that modulating water quality parameters may serve as a viable strategy to mitigate the eco-environmental risk of ARGs in aquaculture water. This study identified antibiotic resistance characteristics in freshwater aquaculture ecosystems of Micropterus salmoides in Zhejiang Province, establishing a foundation on managing antibiotic resistance risks in such aquaculture environments.
水产养殖生态系统中的残留抗生素会对细菌群落施加选择压力，促使细菌通过基因突变或水平基因转移（HGT）获得抗生素抗性基因（ARGs）。本研究调查了浙江省淡水养殖生态系统中大口黑鲈的抗生素抗性风险。结果显示，土霉素、环丙沙星和氟苯尼考含量高达300 ng/L，在所调查的养殖水体中，多重耐药基因的比例在32.20%至50.70%之间。此外，所有注释的ARGs中约9.80%被确定具有质粒介导的水平转移风险。ARGs宿主预测显示，放线菌携带的ARGs丰度最高，高达159.38（覆盖度，×/Gb）。此外，Paer_emrE、ksgA、ompR和golS的丰度与叶绿素a浓度呈正相关（p < 0.05），表明藻华可能促进ARGs的进化和转移。ARGs丰度与总磷、总氮、pH值、电导率之间的相关性表明，调节水质参数可能是降低养殖水体中ARGs生态环境风险的可行策略。本研究确定了浙江省大口黑鲈淡水养殖生态系统中的抗生素抗性特征，为管理此类养殖环境中的抗生素抗性风险奠定了基础。
