Zhao Fangfang, Wu Shifeng, Shen Xiuying, Huang Yixin, Zhang Bing, Luo Zhengzhong, Wu Dan, Shen Liuhong, Cao Suizhong, Wang Yue, Zhang Jun, Yu Shumin
College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China.
Qinghai Academy of Animal Science and Veterinary, Qinghai University, Xining 810016, China.
J Hazard Mater. 2025 Aug 15;494:138476. doi: 10.1016/j.jhazmat.2025.138476. Epub 2025 May 8.
The Qinghai-Tibet Plateau (QTP) and yaks play respectively vital roles in global and plateau ecosystems. Antibiotic resistance is a global threat to public health, with antibiotic resistance genes (ARGs) being one of the emerging contaminants. However, few studies have investigated the abundance and diversity of ARGs and mobile genetic elements (MGEs) in the yak upper respiratory tract and their surrounding pastures. Moreover, the possible pathways for ARG transmission within these ecosystems have not yet been elucidated. Therefore, we investigated the ARG profiles, MGE profiles, and ARG-carrying host bacteria in yaks and their pasture collected from Ganzi and Aba region in Sichuan Province. Metagenomic analyses showed that 22 ARG types and 5 MGEs types were identified in 18 samples. Multidrug resistance gene (mexT) and bacitracin resistance gene (bacA) was identiffed as hotspots, which may compromise medical treatment options. Co-occurrence network analysis revealed that 12 bacterial genera may be potential hosts at the genus level. The enrich of ARGs and MGEs diversity were observed in QTP (Sichuan province) pasture ecosystems which demands evidence-based interventions to mitigate ARGs transmission risks.
青藏高原（QTP）和牦牛分别在全球生态系统和高原生态系统中发挥着至关重要的作用。抗生素耐药性是对公共卫生的全球威胁，抗生素耐药基因（ARGs）是新出现的污染物之一。然而，很少有研究调查牦牛上呼吸道及其周围牧场中ARGs和移动遗传元件（MGEs）的丰度和多样性。此外，这些生态系统中ARG传播的可能途径尚未阐明。因此，我们调查了从四川省甘孜和阿坝地区采集的牦牛及其牧场中的ARG谱、MGE谱和携带ARG的宿主细菌。宏基因组分析表明，在18个样本中鉴定出22种ARG类型和5种MGE类型。多药耐药基因（mexT）和杆菌肽耐药基因（bacA）被确定为热点，这可能会影响医疗选择。共现网络分析表明，12个细菌属可能是属水平上的潜在宿主。在青藏高原（四川省）牧场生态系统中观察到ARGs和MGEs多样性的富集，这需要基于证据的干预措施来降低ARGs传播风险。
