Tikhomirova Maria A, Zharikova Anastasia A, Musinova Yana R, Sheval Eugene V
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119991, Russia.
Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, 119334, Russia.
Histochem Cell Biol. 2025 May 17;163(1):53. doi: 10.1007/s00418-025-02387-0.
Immunocytochemistry and immunohistochemistry are among the most widely used and valuable methods for localizing and quantifying proteins in cells and tissues. However, these methods have several limitations, and they are still being improved. A notable problem in this area is the restricted accessibility of some dense intracellular structures, such as nucleoli, to specific antibodies. This reduced accessibility led to peripheral staining of the nucleoli even in cases of relatively homogeneous distribution of the antigen within the nucleoli. This review aims to elucidate the factors impeding antibody accessibility to the internal regions of the nucleolus in somatic cells and nucleolus-like bodies in oocytes and to explore the methods used to circumvent this limitation.
免疫细胞化学和免疫组织化学是在细胞和组织中定位和定量蛋白质最广泛使用且有价值的方法之一。然而，这些方法存在一些局限性，并且仍在不断改进。该领域一个值得注意的问题是某些致密的细胞内结构（如核仁）对特定抗体的可及性受限。这种可及性降低导致即使在抗原在核仁内分布相对均匀的情况下，核仁也会出现外周染色。这篇综述旨在阐明阻碍抗体进入体细胞内核仁内部区域以及卵母细胞内核仁样体的因素，并探索用于规避这一局限性的方法。
