通过磷光寿命成像对视网膜氧合和代谢进行定量分析。
Quantifying retinal oxygenation and metabolism by phosphorescence lifetime imaging.
作者信息
Shahidi Mahnaz
机构信息
Department of Ophthalmology, University of Southern California Keck School of Medicine, Los Angeles, CA, USA; Alfred E. Mann Department of Biomedical Engineering, University of Southern California, Los Angeles, CA, USA.
出版信息
Exp Eye Res. 2025 Aug;257:110422. doi: 10.1016/j.exer.2025.110422. Epub 2025 May 15.
The retina is a highly metabolically active tissue, requiring adequate availability of oxygen and other metabolites to generate energy for cellular survival and visual function. Retinal hypoxia has been implicated in several common retinal diseases and associated with the development of vision-threatening pathologies. Since the level of hypoxia determines processes that are activated for either cell survival or death, knowledge of retinal oxygenation is essential. This article reviews depth-resolved quantitative measurements of retinal vascular and tissue oxygen tension in rodents using the technique of phosphorescence lifetime imaging. Furthermore, retinal oxygen metabolic biomarkers were quantitatively derived from oxygen tension measurements and shown to be altered under challenged physiological and pathological conditions. Application of phosphorescence lifetime imaging can be useful for advancing knowledge of retinal ischemia pathophysiology and identifying physiological biomarkers to monitor progression and evaluate therapeutic interventions in animal models of human retinal diseases.
视网膜是一种代谢高度活跃的组织，需要充足的氧气和其他代谢物来产生能量，以维持细胞存活和视觉功能。视网膜缺氧与几种常见的视网膜疾病有关，并与威胁视力的病变发展相关。由于缺氧程度决定了细胞存活或死亡所激活的过程，因此了解视网膜氧合情况至关重要。本文综述了利用磷光寿命成像技术对啮齿动物视网膜血管和组织氧张力进行深度分辨定量测量的研究。此外，视网膜氧代谢生物标志物是从氧张力测量中定量得出的，并显示在受到挑战的生理和病理条件下会发生改变。磷光寿命成像的应用有助于增进对视网膜缺血病理生理学的了解，并识别生理生物标志物，以监测人类视网膜疾病动物模型中的病情进展并评估治疗干预措施。
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