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从哥斯达黎加森林中本地豆科植物根瘤分离出的四种潜在慢生根瘤菌属新物种的基因组序列草图。

Draft genome sequences of four potential new species of the genus Bradyrhizobium isolated from root nodules of native legumes in Costa Rican forests.

作者信息

Fernández-Vargas Rachelle, Jiménez-Alpízar Sara, Leandro-Arce Valeria, Mendoza-Guido Bradd, Rojas-Jimenez Keilor

机构信息

Escuela de Biología, Universidad Costa Rica, San José, Costa Rica.

Programa de Posgrado en Microbiología, Parasitología, Química Clínica e Inmunología (PPMPGCI), Universidad Costa Rica, San José, Costa Rica.

出版信息

Access Microbiol. 2025 May 16;7(5). doi: 10.1099/acmi.0.000990.v3. eCollection 2025.

DOI:10.1099/acmi.0.000990.v3
PMID:40385569
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12084545/
Abstract

Here, we report the draft genome sequences of four spp. isolates obtained from root nodules of the native legumes , , and in tropical forests of Costa Rica. Genomes ranged from 8.6 to 9.8 Mb with GC contents between 62.8% and 63.8%. Phylogenomic analysis, along with average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) comparisons, confirmed that these isolates represent potential new species. ANI values ranged from 88.3% to 90.3%, and dDDH values from 28.8% to 41.8%, compared with their closest species. Functional annotation revealed some genes related to nitrogen fixation (, , ) and nodulation capacity (, , ). These results provide insights into the diversity and symbiotic capabilities of in tropical ecosystems.

摘要

在此，我们报告了从哥斯达黎加热带森林中本地豆科植物、、和的根瘤中获得的4种菌株的基因组序列草图。基因组大小在8.6至9.8 Mb之间，GC含量在62.8%至63.8%之间。系统基因组分析以及平均核苷酸同一性（ANI）和数字DNA-DNA杂交（dDDH）比较证实，这些分离株代表潜在的新物种。与它们最接近的物种相比，ANI值在88.3%至90.3%之间，dDDH值在28.8%至41.8%之间。功能注释揭示了一些与固氮（、、）和结瘤能力（、、）相关的基因。这些结果为热带生态系统中的多样性和共生能力提供了见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee16/12084545/cf68ff40cc61/acmi-7-00990-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee16/12084545/c2aaa063f13b/acmi-7-00990-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee16/12084545/cf68ff40cc61/acmi-7-00990-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee16/12084545/c2aaa063f13b/acmi-7-00990-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee16/12084545/cf68ff40cc61/acmi-7-00990-g002.jpg

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