Draft genome sequence of type strain HBR26 and description of sp. nov.

sp. nov. is a newly proposed species within the genus . This species includes six rhizobial strains; which were isolated from root nodules of the legume plant growing in soils of Ethiopia. The species fixes nitrogen effectively in symbiosis with the host plant and is composed of aerobic, Gram-negative staining, rod-shaped bacteria. The genome of type strain HBR26 of sp. nov. was one of the rhizobial genomes sequenced as a part of the DOE JGI 2014 Genomic Encyclopedia project designed for soil and plant-associated and newly described type strains. The genome sequence is arranged in 62 scaffolds and consists of 6,557,588 bp length, with a 61% G + C content and 6221 protein-coding and 86 RNAs genes. The genome of HBR26 contains genes (plasmid replication genes) homologous to the genes found in five different CFN42 plasmids, suggesting that HBR26 may have five additional replicons other than the chromosome. In the genome of HBR26, the nodulation genes and are located in the same module, and organized in a similar way as genes found in the genome of other known common bean-nodulating rhizobial species. gene is found in a different scaffold, but it is also very similar to genes of other bean-nodulating rhizobial strains. Though HBR26 is distinct on the phylogenetic tree and based on ANI analysis (the highest value 90.2% ANI with CFN42) from other bean-nodulating species, these genes and most nitrogen-fixing genes found in the genome of HBR26 share high identity with the corresponding genes of known bean-nodulating rhizobial species (96-100% identity). This suggests that symbiotic genes might be shared between bean-nodulating rhizobia through horizontal gene transfer. sp. nov. was grouped into the genus but was distinct from all recognized species of that genus by phylogenetic analyses of combined sequences of the housekeeping genes and . The closest reference type strains for HBR26 were CFN42 (94% similarity of the combined and sequences) and BLR175 (93%). Genomic ANI calculation based on protein-coding genes also revealed that the closest reference strains were BLR175 and CFN42 with ANI values 91.8 and 90.2%, respectively. Nevertheless, the ANI values between HBR26 and BLR175 or CFN42 are far lower than the cutoff value of ANI (> = 96%) between strains in the same species, confirming that HBR26 belongs to a novel species. Thus, on the basis of phylogenetic, comparative genomic analyses and ANI results, we formally propose the creation of sp. nov. with strain HBR26 (=HAMBI 3550=LMG 29711) as the type strain. The genome assembly and annotation data is deposited in the DOE JGI portal and also available at European Nucleotide Archive under accession numbers FMAJ01000001-FMAJ01000062.