Pugsley A P, Reeves P
Antimicrob Agents Chemother. 1977 Feb;11(2):345-58. doi: 10.1128/AAC.11.2.345.
Colicins B-K260 and D-CA23 were purified by ammonium sulfate precipitation, gel filtration, and ion-exchange chromatography and were compared with respect to a number of physical and chemical properties. Both colicins were shown to be proteins and were found to have similar molecular weights, isoelectric points and amino acid compositions. The two colicins also have substantial antigenic similarities but are distinguished by the presence of non-cross-reacting antigens and by differences in stability and in sensitivity to heat and reducing conditions. In addition, strains of Escherichia coli K-12 producing colicins B-K260 and D-CA23 are not cross-immune. The similarities noted between the two colicins are compatible with their use of a common cell surface receptor while having different modes of action.
通过硫酸铵沉淀、凝胶过滤和离子交换色谱法纯化了大肠杆菌素B-K260和D-CA23，并对它们的一些物理和化学性质进行了比较。两种大肠杆菌素均显示为蛋白质，且发现它们具有相似的分子量、等电点和氨基酸组成。这两种大肠杆菌素在抗原性上也有很大相似性，但可通过存在非交叉反应抗原以及稳定性、对热和还原条件的敏感性差异来区分。此外，产生大肠杆菌素B-K260和D-CA23的大肠杆菌K-12菌株没有交叉免疫性。两种大肠杆菌素之间的相似性与其使用共同的细胞表面受体但作用方式不同是相符的。
