Bronaugh R L, Stewart R F, Wester R C, Bucks D, Mailbach H I, Anderson J
Food Chem Toxicol. 1985 Jan;23(1):111-4. doi: 10.1016/0278-6915(85)90228-5.
The percutaneous absorption of two cosmetic fragrance materials, safrole and cinnamyl anthranilate, as well as of cinnamic alcohol and cinnamic acid, has been measured at occluded and non-occluded application sites. Absorption values were determined in the rhesus monkey in vivo. Absorption through human skin was measured by using excised skin in diffusion cells. Because of the insolubility in water of safrole and cinnamyl anthranilate, a nonionic surfactant solution (6% oleth 20) was used in the receptor chamber of the diffusion cell in order to facilitate the partitioning of the compounds from the skin into the receptor fluid. The relative volatility of the compounds was determined in order to aid in the interpretation of the absorption results. The greatest difference between in vivo and in vitro absorption values occurred with safrole, which was the least well absorbed and the most volatile compound. Cinnamic acid absorption through non-occluded human skin (17.8 +/- 4.9%, mean +/- SEM) was significantly lower than through monkey skin (38.6 +/- 8.3%). The values for absorption through human and monkey skin did not differ significantly for cinnamyl anthranilate (24.0 +/- 5.1% v. 26.1 +/- 2.3%) or cinnamic alcohol (33.9 +/- 7.3% v. 25.4 +/- 4.4%). Occlusion of the skin resulted in greater permeation of all of the compounds; a significant difference in permeability between the two types of skin occurred only with safrole. The fragrances were absorbed well, but their volatility must be considered in a toxicity evaluation. There was reasonable agreement between the values obtained from the studies of the human skin in vitro and the monkey skin in vivo.
已在封闭和非封闭应用部位测量了两种化妆品香料成分黄樟素和邻氨基苯甲酸肉桂酯以及肉桂醇和肉桂酸的经皮吸收情况。吸收值是在恒河猴体内测定的。通过使用扩散池中切除的皮肤来测量其在人皮肤上的吸收情况。由于黄樟素和邻氨基苯甲酸肉桂酯在水中不溶，因此在扩散池的接受室中使用了非离子表面活性剂溶液（6%油醇醚20），以促进化合物从皮肤向接受液的分配。测定了这些化合物的相对挥发性，以辅助解释吸收结果。体内和体外吸收值之间的最大差异出现在黄樟素上，它是吸收最差且挥发性最高的化合物。肉桂酸在非封闭人皮肤上的吸收（17.8±4.9%，平均值±标准误）显著低于在猴皮肤上的吸收（38.6±8.3%）。对于邻氨基苯甲酸肉桂酯（24.0±5.1%对26.1±2.3%）或肉桂醇（33.9±7.3%对25.4±4.4%），人皮肤和猴皮肤的吸收值没有显著差异。皮肤封闭会导致所有化合物的渗透增加；仅黄樟素在两种皮肤类型之间的渗透率存在显著差异。这些香料吸收良好，但在毒性评估中必须考虑它们的挥发性。体外人皮肤研究和体内猴皮肤研究获得的值之间存在合理的一致性。
