Evans L, Mitchison T, Kirschner M
J Cell Biol. 1985 Apr;100(4):1185-91. doi: 10.1083/jcb.100.4.1185.
The capacity of the centrosome to influence the lattice structure of nucleated microtubules was studied in vitro. Brain microtubules self-assembled to give predominantly (98%) 14-protofilament microtubules. However, under exactly the same conditions of assembly they grew off of purified centrosomes from neuroblastoma cells to give mostly (82%) 13-protofilament microtubules. Thus, the nucleation sites on the centrosome constrained the microtubule lattice to yield the number of protofilaments usually found in vivo.
在体外研究了中心体影响有核微管晶格结构的能力。脑微管自组装后主要产生（98%）14根原纤维的微管。然而，在完全相同的组装条件下，它们从神经母细胞瘤细胞的纯化中心体上生长，大多产生（82%）13根原纤维的微管。因此，中心体上的成核位点限制了微管晶格，以产生通常在体内发现的原纤维数量。
