Seo Ji Won, Ham Da Ye, Lee Jae Geun, Kim Hee Young, Choi Ik Young, Kim Myong Jo, Seong Eun Soo
Interdisciplinary Program in Smart Science, Kangwon National University, Chuncheon, 24341, Republic of Korea.
Research Institute of Biotechnology, Hwajin Cosmetics, Hongcheon, 25142, Republic of Korea.
BMC Genomics. 2025 May 19;26(1):506. doi: 10.1186/s12864-025-11720-9.
Plant basic transcription factor3 (BTF3) plays an important role in photosynthesis rate and plant growth and development. In addition, it is involved in resistance mechanisms related to abiotic and biotic stress and has a significant impact on plant growth phenotype. This study was conducted to expand our understanding on plant transcriptome changes as there are no reports on plant transcriptome changes in normal environments due to the overexpression of Miscanthus sinensis BTF3 gene.
The amino acid sequence length of the BTF3 gene isolated from M. sinensis was 158 aa, and it showed the highest homology (97.47 %) with that of Paniocum virgatum (PvBTF3). Nicotiana benthamiana (Nb) transgenic plant was produced by transforming a binary vector into which the MsBTF3 gene was inserted into Agrobacterium. Up- and down-regulatory factors were classified through transcriptome analysis using transgenic plants overexpressing MsBTF3. Gene ontology (GO) analysis of up-regulated transcripts showed that the most expressed transcripts were involved in biological processes. Analysis of down-regulated differentially expressed genes (DEGs) showed that they were involved in metabolic processes that corresponded to biological processes. Blast analysis revealed that Nb03180T (chloroplast photosystem II 22 kDa component), Nb04871T (basic transcription factor 3-like), Nb13433T (expansin-B15- like), Nb15392T (receptor-like serine/threonine-protein kinase SD1-8 isoform X3), Nb17216T (3-bata-glucan endohydrolase), and Nb20214T (probable rhamnogalacturonate lyase B) were among the DEGs whose transcript expression was up-regulated more than 2-fold compared to the wild type. In particular, the reference gene, that is the NbBTF3 gene, showed the highest expression owing to the overexpression of MsBTF3.
Transcriptome changes in tobacco plants through MsBTF3 overexpression revealed that MsBTF3 caused broad-spectrum changes in the biological processes of plants. MsBTF3 showed that various metabolic and defense responses could be activated by regulating the level of specific gene expression in plants. These data obtained through this process will greatly contribute to elucidating the mechanism by which MsBTF3 helps strong physiological responses in plants.
植物基础转录因子3（BTF3）在光合速率以及植物生长发育过程中发挥着重要作用。此外，它还参与了与非生物和生物胁迫相关的抗性机制，对植物生长表型有显著影响。由于尚无关于芒草BTF3基因过表达导致正常环境下植物转录组变化的报道，本研究旨在拓展我们对植物转录组变化的认识。
从芒草中分离得到的BTF3基因的氨基酸序列长度为158个氨基酸，与柳枝稷（PvBTF3）的氨基酸序列具有最高的同源性（97.47%）。通过将插入了MsBTF3基因的二元载体转化到农杆菌中，培育出了本氏烟草（Nb）转基因植株。利用过表达MsBTF3的转基因植株进行转录组分析，对上调和下调因子进行了分类。对上调转录本的基因本体（GO）分析表明，表达量最高的转录本参与了生物过程。对下调的差异表达基因（DEG）的分析表明，它们参与了与生物过程相对应的代谢过程。Blast分析显示，Nb03180T（叶绿体光系统II 22 kDa组分）、Nb04871T（类基础转录因子3）、Nb13433T（类扩张蛋白-B15）、Nb15392T（类受体丝氨酸/苏氨酸蛋白激酶SD1-8亚型X3）、Nb17216T（3-β-葡聚糖内切酶）和Nb20214T（可能的鼠李糖半乳糖醛酸裂解酶B）是转录表达相对于野生型上调超过2倍的DEG。特别是，参考基因即NbBTF3基因，由于MsBTF3的过表达而表现出最高的表达量。
通过MsBTF3过表达导致烟草植株转录组变化，表明MsBTF3引起了植物生物过程的广谱变化。MsBTF3表明，通过调节植物中特定基因的表达水平，可以激活各种代谢和防御反应。通过这一过程获得的这些数据将极大地有助于阐明MsBTF3帮助植物产生强烈生理反应的机制。
