Milvae R A, Hansel W
J Anim Sci. 1985 Feb;60(2):528-31. doi: 10.2527/jas1985.602528x.
Experiments were conducted to determine the effects of infusing indomethacin, a prostaglandin synthetase inhibitor, into the uterine lumen on the development and function of the bovine corpus luteum in the presence and absence of concurrently administered oxytocin. Each treatment was given twice daily on d 4, 5 and 6 of the estrous cycle. Treatments (six heifers/group) and resulting estrous cycle lengths were as follows: (1) untreated controls, 20.6 +/- .4 d; (2) .2 M phosphate buffer vehicle infused into the uterine lumen, 21.0 +/- .6 d; (3) 40 mg indomethacin infused into the body of the uterus, 16.5 +/- 1.0 d; (4) 150 USP units oxytocin injected sc, 10.0 +/- 1.2 d and (5) a combination of oxytocin and indomethacin as in treatments 3 and 4, 14.1 +/- 1.3 d. Plasma concentrations of progesterone were lower (P less than .05) in each treatment group from d 7 onward, when compared with untreated and vehicle-treated controls. Indomethacin alone effectively inhibited the development and function of the corpus luteum, and was without effect on oxytocin-induced inhibition of luteal function. In summary, it appears that a prostaglandin of either uterine or ovarian origin, or both, is required for the normal development and function of the bovine corpus luteum.
进行了实验，以确定在同时给予或不给予催产素的情况下，将前列腺素合成酶抑制剂吲哚美辛注入子宫腔对牛黄体发育和功能的影响。在发情周期的第4、5和6天，每天对每种处理进行两次给药。处理方式（每组6头小母牛）及由此产生的发情周期长度如下：（1）未处理的对照组，20.6±0.4天；（2）向子宫腔内注入0.2M磷酸盐缓冲液载体，21.0±0.6天；（3）向子宫体注入40mg吲哚美辛，16.5±1.0天；（4）皮下注射150USP单位催产素，10.0±1.2天；（5）如处理3和4那样联合使用催产素和吲哚美辛，14.1±1.3天。从第7天起，与未处理和载体处理的对照组相比，每个处理组的血浆孕酮浓度均较低（P<0.05）。单独使用吲哚美辛可有效抑制黄体的发育和功能，且对催产素诱导的黄体功能抑制无影响。总之，看来子宫或卵巢来源的前列腺素，或两者均需，才能使牛黄体正常发育和发挥功能。
