Thibodeaux J K, Myers M W, Roussel J D, Godke R A
Department of Dairy Science, Louisiana State University, Baton Rouge 70803.
Prostaglandins. 1992 Dec;44(6):531-41. doi: 10.1016/0090-6980(92)90023-m.
The objective was to evaluate the effect of intrauterine infusion of prostaglandin E2 (PGE2) on luteal function in cattle. Heifers and cows were randomly assigned after two normal estrous cycles to either PGE2 or control treatment groups. Females in Treatment A were infused with 1 mg of PGE2 once daily into the uterine horn ipsilateral to the corpus luteum between days 7-10 of the estrous cycle with a 0.25 ml plastic semen straw and an artificial insemination pipette. Females in Treatment B were similarly infused with 1 mg of PGE2 once daily in 20 ml of a carrier vehicle via a catheter on days 10 and 11 of the estrous cycle. Control animals were infused with the carrier vehicle using either a semen straw (Treatment C) or via a catheter (Treatment D) on the same days of the estrous cycle. Blood samples were collected daily to monitor plasma progesterone concentrations during the treatment period. Females infused with PGE2 on days 7-10 of the estrous cycle returned to estrus in a mean of 23.5 days (range 22-25 days) and were similar (P > 0.05) to those infused on days 10 and 11 which returned to estrus in 23.5 days (range 22-25 days). Animals similarly infused with carrier vehicle on the same days of the estrous cycle returned to standing estrus in 20.2 days (range 17-23 days). Plasma progesterone concentrations indicated an extended period of elevated progesterone concentrations in PGE2-treated animals compared with control animals. These results indicate that short term administration of PGE2 early in the estrous cycle may result in extended luteal maintenance.
目的是评估子宫内注入前列腺素E2（PGE2）对母牛黄体功能的影响。在经历两个正常发情周期后，将小母牛和母牛随机分为PGE2处理组或对照组。处理A组的雌性动物在发情周期的第7至10天，使用0.25毫升塑料精液吸管和人工授精移液管，每天一次向黄体同侧子宫角注入1毫克PGE2。处理B组的雌性动物在发情周期的第10和11天，通过导管每天一次在20毫升载体溶液中注入1毫克PGE2。对照动物在发情周期的同一天，使用精液吸管（处理C）或通过导管（处理D）注入载体溶液。在治疗期间每天采集血样以监测血浆孕酮浓度。在发情周期第7至10天注入PGE2的雌性动物平均在23.5天（范围22 - 25天）恢复发情，与在第10和11天注入PGE2且在23.5天（范围22 - 25天）恢复发情的动物相似（P > 0.05）。在发情周期同一天同样注入载体溶液的动物在20.2天（范围17 - 23天）恢复静立发情。与对照动物相比，PGE2处理动物的血浆孕酮浓度显示孕酮浓度升高的时间延长。这些结果表明，在发情周期早期短期给予PGE2可能导致黄体维持时间延长。
