Johnson D W, McEvoy M, Seamark R F, Cox L W, Phillipou G
J Steroid Biochem. 1985 Mar;22(3):349-53. doi: 10.1016/0022-4731(85)90437-6.
A method employing stable isotope-labelled tracers and gas chromatograph-mass spectrometry (GC-MS) analysis has been used to measure the plasma clearance rates (PCR's) of androstenedione (A) and testosterone (T) in normal women and women with androgen abnormalities including hirsutism and polycystic ovary syndrome. A solution of deuterium-labelled A and T is infused at a constant rate and blood samples taken at 2 and 2.25 h. Solvent extracts of the derived plasma samples, to which an internal standard has been added, are derivatized with pentafluoropropionic anhydride and the endogenous steroid and deuterated steroid are quantitated after an injection of the derivatization mixture into a capillary column GC-MS. The concentration of the deuterated steroid in the infusion mixture is measured and the PCR is calculated. In premenopausal normal women the PCRA is 1950 +/- 184 1/24 h (n = 5) and the PCRT is 484 +/- 82 1/24 h (n = 7).
一种采用稳定同位素标记示踪剂和气相色谱 - 质谱联用（GC - MS）分析的方法，已用于测量正常女性以及患有雄激素异常（包括多毛症和多囊卵巢综合征）的女性中雄烯二酮（A）和睾酮（T）的血浆清除率（PCR）。以恒定速率输注氘标记的A和T溶液，并在2小时和2.25小时采集血样。向所得血浆样品的溶剂提取物中加入内标，用五氟丙酸酐进行衍生化处理，将衍生化混合物注入毛细管柱GC - MS后，对内源性类固醇和氘代类固醇进行定量。测量输注混合物中氘代类固醇的浓度并计算PCR。在绝经前正常女性中，PCRA为1950±184 1/24小时（n = 5），PCRT为484±82 1/24小时（n = 7）。
