Li Zi-Dong, Yao Xin-Li, Zhang Lingqian, Zhang Jiajing, Hao Jian-Hong, Wu Huashuang, Dong Shuqi, Yuan Xiangyang, Li Xiaorui, Gao Lulu, Yang Guanghui, Chu Xiaoqian, Wang Jia-Gang, Wu Yuxiang
College of Agriculture, Shanxi Agricultural University, Taigu, 030801, China.
Hou Ji Laboratory in Shanxi Province, Shanxi Agricultural University, Taigu, 030801, China.
Planta. 2025 May 20;262(1):2. doi: 10.1007/s00425-025-04722-0.
The molecular mechanism of cleistogamy in foxtail millet was analyzed. We found that changes of cell wall components caused the abnormal development of the lodicule. Outcrossing between cultivated crops and their associated wild species may result in the loss of favorable agricultural traits in the progeny or the drift of genetically modified (GM) organisms in future generations. In this study, we found a cleistogamy mutant with an abnormal development of the lodicules. The lodicules were sampled when the florets just to bloom for RNA-seq analysis. Our results showed that, compared with the wild type, the mutant had more enriched pathways related to cell wall synthesis or decomposition. Staining of the lodicule cell wall showed that the mutant had relatively lower amounts of callose and cellulose than the wild type. The content of pectin methylation in the lodicule cell wall of the mutant was lower than that of the wild type. In summary, we analyzed the causes of cleistogamy phenotype in foxtail millet. Transcriptome data analysis revealed that this was probably caused by dysplasia of the lodicule cell wall. Cytochemical staining and immunofluorescence tests showed that the changes of the components in the cell wall caused the abnormal development of the lodicule. Overall, this study sheds light on the potential molecular mechanism of cleistogamy of foxtail millet and provides a theoretical basis for subsequent research.
分析了谷子闭花受精的分子机制。我们发现细胞壁成分的变化导致浆片发育异常。栽培作物与其相关野生种之间的杂交可能导致后代优良农艺性状的丧失或转基因生物在后代中的漂移。在本研究中，我们发现了一个浆片发育异常的闭花受精突变体。在小花即将开放时采集浆片进行RNA测序分析。我们的结果表明，与野生型相比，突变体中与细胞壁合成或分解相关的途径更为富集。浆片细胞壁染色显示，突变体的胼胝质和纤维素含量低于野生型。突变体浆片细胞壁中果胶甲基化含量低于野生型。综上所述，我们分析了谷子闭花受精表型的原因。转录组数据分析表明，这可能是由于浆片细胞壁发育异常所致。细胞化学染色和免疫荧光试验表明，细胞壁成分的变化导致浆片发育异常。总体而言，本研究揭示了谷子闭花受精的潜在分子机制，为后续研究提供了理论依据。
