海胆卵皂苷模型中的裂解

Cleavage in a saponin model of the sea urchin egg.

作者信息

Yoshimoto Y, Hiramoto Y

出版信息

Cell Struct Funct. 1985 Mar;10(1):29-36. doi: 10.1247/csf.10.29.

DOI:10.1247/csf.10.29

Abstract

A cell model, in which cleavage could be induced, was obtained from fertilized sea urchin eggs by putting eggs that were in the first cleavage into a solution containing 3 X 10(-5) g/ml saponin and suitable amounts of ATP and Ca2+. The cell membrane became freely permeable to ATP and Ca2+ within 1 minute. The respective optimal concentrations of ATP and Ca2+ that advanced the cleavage furrow in this model were 2 mM and 10(-8) M. With the optimal ATP and Ca2+ concentrations, the cleavage furrow of the model advanced at a rate that differed little from that in living eggs. The cleavage furrow soon receded, however, when the concentration of ATP was decreased to less than 1 mM or increased to more than 3 mM, as well as when the concentration of Ca2+ was increased to more than 10(-7) M.

摘要

通过将处于第一次卵裂期的海胆受精卵放入含有3×10⁻⁵克/毫升皂角苷以及适量ATP和Ca²⁺的溶液中，获得了一种能够诱导卵裂的细胞模型。细胞膜在1分钟内对ATP和Ca²⁺变得可自由通透。在该模型中推进卵裂沟的ATP和Ca²⁺的各自最佳浓度分别为2毫摩尔/升和10⁻⁸摩尔/升。在最佳ATP和Ca²⁺浓度下，该模型的卵裂沟推进速度与活卵中的推进速度相差不大。然而，当ATP浓度降至低于1毫摩尔/升或升至高于3毫摩尔/升时，以及当Ca²⁺浓度升至高于10⁻⁷摩尔/升时，卵裂沟很快就会后退。