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Dephosphorylation suppresses the activity of neurofilament to promote tubulin polymerization.

作者信息

Minami Y, Sakai H

出版信息

FEBS Lett. 1985 Jun 17;185(2):239-42. doi: 10.1016/0014-5793(85)80914-5.

DOI:10.1016/0014-5793(85)80914-5
PMID:4039683
Abstract

The phosphate content of neurofilament was diminished by half, from 49.4 to 22.9 nmol/mg, by treatment with alkaline phosphatase. Dephosphorylation decreased the activity of neurofilament to promote tubulin assembly. This suppression was supposed to be mainly due to dephosphorylation of the 200 kDa subunit of neurofilament. Dephosphorylation of the isolated 200 kDa subunit caused suppression of its activity to promote tubulin polymerization. These results suggest that changes in the phosphate content modulate interaction of neurofilament with tubulin.

摘要

相似文献

1
Dephosphorylation suppresses the activity of neurofilament to promote tubulin polymerization.
FEBS Lett. 1985 Jun 17;185(2):239-42. doi: 10.1016/0014-5793(85)80914-5.
2
Network formation by neurofilament-induced polymerization of tubulin: 200K subunit of neurofilament triplet promotes nucleation of tubulin polymerization and enhances microtubule assembly.神经丝诱导微管蛋白聚合形成网络：神经丝三联体的200K亚基促进微管蛋白聚合的成核作用并增强微管组装。
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3
Participation of 200K or 150K subunit of neurofilament in construction of the filament core with 70K subunit and promotion of tubulin polymerization by incorporated 200K subunit.神经丝200K或150K亚基参与由70K亚基构成的丝状体核心的构建，以及由掺入的200K亚基促进微管蛋白聚合。
J Biochem. 1984 Nov;96(5):1481-90. doi: 10.1093/oxfordjournals.jbchem.a134977.
4
Dephosphorylation of neurofilaments by exogenous phosphatases has no effect on reassembly of subunits.外源性磷酸酶对神经丝的去磷酸化作用对亚基的重新组装没有影响。
J Neurochem. 1986 Aug;47(2):477-83. doi: 10.1111/j.1471-4159.1986.tb04526.x.
5
Interaction of tubulin with neurofilaments: formation of networks by neurofilament-dependent tubulin polymerization.微管蛋白与神经丝的相互作用：依赖神经丝的微管蛋白聚合形成网络。
J Biochem. 1982 Sep;92(3):889-98. doi: 10.1093/oxfordjournals.jbchem.a134003.
6
The susceptibility of MAP-2 to proteolytic degradation increases when bound to tubulin.
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7
Phosphorylation of beta III-tubulin.βIII-微管蛋白的磷酸化
Biochemistry. 1996 Mar 26;35(12):3704-11. doi: 10.1021/bi951247p.
8
Influence of the phosphorylation state of neurofilament proteins on the interactions between purified filaments in vitro.神经丝蛋白磷酸化状态对体外纯化丝之间相互作用的影响。
Biochem J. 1988 Jun 15;252(3):655-60. doi: 10.1042/bj2520655.
9
An isoelectric variant of the 150,000-dalton neurofilament polypeptide. Evidence that phosphorylation state affects its association with the filament.150,000道尔顿神经丝多肽的一种等电变体。磷酸化状态影响其与神经丝结合的证据。
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10
Effects of microtubule-associated proteins on network formation by neurofilament-induced polymerization of tubulin.微管相关蛋白对神经丝诱导的微管蛋白聚合形成网络的影响。
FEBS Lett. 1986 Jan 20;195(1-2):68-72. doi: 10.1016/0014-5793(86)80132-6.

引用本文的文献

1
cdc2-like kinase from rat spinal cord specifically phosphorylates KSPXK motifs in neurofilament proteins: isolation and characterization.来自大鼠脊髓的细胞周期蛋白依赖性激酶2样激酶特异性磷酸化神经丝蛋白中的KSPXK基序：分离与鉴定。
Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6844-8. doi: 10.1073/pnas.90.14.6844.
2
Phosphorylation of native and reassembled neurofilaments composed of NF-L, NF-M, and NF-H by the catalytic subunit of cAMP-dependent protein kinase.由NF-L、NF-M和NF-H组成的天然及重新组装的神经丝被环磷酸腺苷依赖性蛋白激酶催化亚基磷酸化。
Mol Biol Cell. 1994 Feb;5(2):161-72. doi: 10.1091/mbc.5.2.161.
3
The human mid-size neurofilament subunit: a repeated protein sequence and the relationship of its gene to the intermediate filament gene family.
人类中分子量神经丝亚基：一种重复的蛋白质序列及其基因与中间丝基因家族的关系。
EMBO J. 1987 Jun;6(6):1617-26. doi: 10.1002/j.1460-2075.1987.tb02409.x.
4
Influence of the phosphorylation state of neurofilament proteins on the interactions between purified filaments in vitro.神经丝蛋白磷酸化状态对体外纯化丝之间相互作用的影响。
Biochem J. 1988 Jun 15;252(3):655-60. doi: 10.1042/bj2520655.
5
Expression of NF-L and NF-M in fibroblasts reveals coassembly of neurofilament and vimentin subunits.成纤维细胞中神经丝轻链蛋白（NF-L）和神经丝中链蛋白（NF-M）的表达揭示了神经丝和波形蛋白亚基的共同组装。
J Cell Biol. 1989 Feb;108(2):579-93. doi: 10.1083/jcb.108.2.579.
6
Characterization of dominant and recessive assembly-defective mutations in mouse neurofilament NF-M.小鼠神经丝蛋白NF-M中显性和隐性装配缺陷突变的特征分析
J Cell Biol. 1990 Nov;111(5 Pt 1):1987-2003. doi: 10.1083/jcb.111.5.1987.
7
Expression of NF-L in both neuronal and nonneuronal cells of transgenic mice: increased neurofilament density in axons without affecting caliber.神经丝轻链（NF-L）在转基因小鼠的神经元和非神经元细胞中的表达：轴突中神经丝密度增加而不影响轴突管径。
J Cell Biol. 1990 Oct;111(4):1543-57. doi: 10.1083/jcb.111.4.1543.
8
Effects of phosphorylation of the neurofilament L protein on filamentous structures.神经丝L蛋白磷酸化对丝状结构的影响。
Cell Regul. 1990 Jan;1(2):237-48. doi: 10.1091/mbc.1.2.237.