Karaffová Viera, Szabóová Renáta, Tóthová Csilla, Žitňan Rudolf, Čechová Michaela, Levkut Martin, Hudec Erik, Ševčíková Zuzana, Röntgen Monika, Albrecht Elke, Herich Róbert
Department of Morphological Disciplines, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovakia.
Department of Biology and Physiology, University of Veterinary Medicine in Košice, Košice, Slovak Republic.
Vet Res Commun. 2025 May 21;49(4):207. doi: 10.1007/s11259-025-10769-x.
Despite the growing interest in the use of probiotics in broilers as feed additives, studies conducted to investigate the effect of probiotic administration on acute phase responses and the impact on muscle growth parameters in broilers are still limited. In this study, we investigated the effect of Bacillus amyloliquefaciens CECT 5940 on relative gene expression for growth factors involved in muscle development (insulin-like growth factors, myogenic factor 5, paired-box transcription factor), percentage of proliferating antigen cell nuclei in breast muscle, and secretion of acute phase proteins (serum amyloid A, haptoglobin, alpha-acid glycoprotein) in the peripheral blood of broilers. Sixty one-day-old chicks from the experimental group were sprayed with a probiotic containing B. amyloliquefaciens at a dose of 1 × 10 CFU/g directly after hatching and received the probiotic in drinking water (50 × 10 CFU/1000 L) for 5 consecutive days of life. Sampling was performed on the 5th, 8th, and 12th day of life of the chicks. From the obtained results, we can conclude that B. amyloliquefaciens modulated the gene expression of selected growth parameters in the pectoral muscle, thereby increasing the number of satellite cells, however, their uptake into muscle fibers and thus increased hypertrophic growth was not proven at the time of the last sampling. At the same time, it demonstrated a potentiating effect on PCNA expression in chicken breast muscles in the early intensive growth phase of broiler chickens and modulated the production of acute phase proteins, which may contribute to improving adaptive processes during the growth and development of broilers.
尽管人们对将益生菌用作肉鸡饲料添加剂的兴趣日益浓厚，但关于益生菌给药对肉鸡急性期反应的影响以及对肌肉生长参数的影响的研究仍然有限。在本研究中，我们调查了解淀粉芽孢杆菌CECT 5940对参与肌肉发育的生长因子（胰岛素样生长因子、生肌因子5、配对盒转录因子）的相对基因表达、胸肌中增殖抗原细胞核的百分比以及肉鸡外周血中急性期蛋白（血清淀粉样蛋白A、触珠蛋白、α-酸性糖蛋白）分泌的影响。实验组的60只1日龄雏鸡在孵化后立即以1×10 CFU/g的剂量喷洒含解淀粉芽孢杆菌的益生菌，并在其生命的连续5天内饮用含有该益生菌的水（50×10 CFU/1000 L）。在雏鸡生命的第5、8和12天进行采样。从获得的结果可以得出结论，解淀粉芽孢杆菌调节了胸肌中选定生长参数的基因表达，从而增加了卫星细胞的数量，然而，在最后一次采样时，并未证实它们被摄取到肌纤维中，因此也没有证明其促进了肥大生长。同时，它在肉鸡早期快速生长阶段对鸡胸肌中PCNA的表达具有增强作用，并调节了急性期蛋白的产生，这可能有助于改善肉鸡生长发育过程中的适应性过程。
