Yu Yang, Li Qing, Zeng Xinfu, Xu Yinglei, Jin Kan, Liu Jinsong, Cao Guangtian
Key Laboratory of Applied Technology on Green-Eco-Health Animal Husbandry of Zhejiang Province, Zhejiang Province Engineering Laboratory for Animal Health and Internet Technology, College of Ani-mal Science and Technology, College of Veterinary Medicine, Zhejiang A & F University, Hangzhou, China.
Zhejiang Vegamax Biotechnology Co., Ltd., Anji, China.
Front Vet Sci. 2022 Feb 21;9:846649. doi: 10.3389/fvets.2022.846649. eCollection 2022.
We aimed to study the effects of dietary () and p () on broilers challenged by lipopolysaccharide (LPS). One-day-old Cobb 500 chicks (360) were divided randomly into three treatment groups for 47 days: no supplementation (control, CON), supplementation (BC), and supplementation (LA). Broilers were routinely fed for 42 days and intraperitoneally injected with 500 μg LPS per kg body weight at 43, 45, and 47 days of age, respectively. Samples were collected 3 h after the last injection. At 1-21 days of age, the ADG in the BC and LA groups was higher than that in the CON group, and the feed to gain ratio (F/G) in the BC group was significantly decreased ( < 0.05). Compared with that in CON birds, the ADG was increased and the F/G was decreased in the BC and LA birds at 22-42 and 1-42 days of age, respectively ( < 0.05). After LPS stimulation, the endotoxin (ET), diamine oxidase (DAO), and D-lactic acid (D-LA) levels in the BC group were lower than those in the CON group ( < 0.05). The IgY, IgA, and IgM contents in the BC group and the IgY and IgM contents in the LA group were higher than those in the CON group ( < 0.05). The pro-inflammatory factor and interferon-β (IFN-β) contents ( < 0.05) decreased, and the anti-inflammatory factor content in the serum ( < 0.05) increased in the BC and LA groups. Compared with the CON and LA treatments, the BC treatment increased the concentrations of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT), and decreased that of malondialdehyde (MDA) ( < 0.05). In contrast with the CON treatment, the BC and LA treatments increased the abundance of and reduced that of ( < 0.05). Moreover, BC increased the abundance of beneficial bacteria. Overall, supplementation with and promoted the growth of broilers, improved their immunity and antioxidant capacity, and alleviated the LPS-stimulated inflammatory response by regulating the intestinal flora.
我们旨在研究日粮（）和（）对受脂多糖（LPS）攻击的肉鸡的影响。将一日龄的科宝500雏鸡（360只）随机分为三个处理组，为期47天：不添加（对照组，CON）、添加（BC）和添加（LA）。肉鸡常规饲养42天，分别在43、45和47日龄时腹腔注射每千克体重500μg LPS。在最后一次注射后3小时采集样本。在1至21日龄时，BC组和LA组的平均日增重高于CON组，且BC组的料重比显著降低（<0.05）。与CON组鸡相比，在22至42日龄和1至42日龄时，BC组和LA组鸡的平均日增重分别增加，料重比降低（<0.05）。LPS刺激后，BC组的内毒素（ET）、二胺氧化酶（DAO）和D - 乳酸（D - LA）水平低于CON组（<0.05）。BC组的IgY、IgA和IgM含量以及LA组的IgY和IgM含量高于CON组（<0.05）。BC组和LA组血清中的促炎因子和干扰素 - β（IFN - β）含量降低（<0.05），抗炎因子含量增加（<0.05）。与CON组和LA组处理相比，BC组处理提高了谷胱甘肽过氧化物酶（GSH - Px）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的浓度，降低了丙二醛（MDA）的浓度（<0.05）。与CON组处理相比，BC组和LA组处理增加了（）的丰度，降低了（）的丰度（<0.05）。此外，BC增加了有益菌的丰度。总体而言，添加（）和（）促进了肉鸡生长，提高了其免疫力和抗氧化能力，并通过调节肠道菌群减轻了LPS刺激的炎症反应。
