Bharati Jaya, Kumar Satish, Buragohain Bijoy Mukut, Das Diptesh, Devi Salam Jayachitra, Mohan N H, Gupta Vivek Kumar
Animal Physiology, ICAR-National Research Centre on Pig, Guwahati, 781131, Assam, India.
Animal Genetics and Breeding, ICAR-National Research Centre on Pig, Guwahati, 781131, Assam, India.
Mol Biol Rep. 2025 May 21;52(1):481. doi: 10.1007/s11033-025-10605-0.
Information on global transcriptomic changes in the porcine ampulla after ovulation is crucial for understanding of oviductal physiology at the molecular level. The objective of the present study was to investigate the differentially expressed genes (DEGs) and signalling pathways regulating the functionality of ampulla in pigs post-ovulation.
The RNA-sequencing of the post-ovulatory ampulla (POA) and early luteal ampulla (ELA) tissues was conducted using Illumina NextSeq2000. The R package NOISeq was used to obtain significantly differentially expressed genes (DEGs) with the probability of differential expression (1-FDR) value ≥ 0.95 and log fold change (logFC) ≥ 1, which revealed 817 DEGs (657 up- and 160 down-regulated) in the POA vs. ELA group comparison. These DEGs were functionally annotated with various gene ontology terms like sterol biosynthetic process, growth, cell migration, and Reactome pathways like signal transduction, metabolism, and cell cycle, indicating key role of these molecular events in POA. The WNT, TNFR2 non-canonical NF-kB, and hedgehog signalling pathways along with the activation of the immune system process, were enriched in the POA vs. ELA group, which indicates their role in cell-cell interactions and cell fate determination in remodelling the oviductal microenvironment during transition from estrogen to progesterone domination. The highly connected upregulated hub genes ESR1, RAD51, YARS1, TYMS and CDK2 can be regarded as key regulatory factors in synchronizing the changes in POA at the molecular level in the oviduct.
The present study revealed several DEGs, signalling pathways and novel modulatory factors associated with the ampullary physiology during early embryonic development in the POA, which may influence fertility and litter size in pigs.
排卵后猪输卵管壶腹部的全球转录组变化信息对于从分子水平理解输卵管生理学至关重要。本研究的目的是调查排卵后猪输卵管壶腹部功能的差异表达基因（DEGs）和信号通路。
使用Illumina NextSeq2000对排卵后壶腹部（POA）和黄体早期壶腹部（ELA）组织进行RNA测序。使用R包NOISeq获得显著差异表达基因（DEGs），差异表达概率（1-FDR）值≥0.95且对数变化倍数（logFC）≥1，这揭示了POA与ELA组比较中有817个DEGs（657个上调和160个下调）。这些DEGs用各种基因本体术语进行功能注释，如甾醇生物合成过程、生长、细胞迁移，以及用Reactome通路进行注释，如信号转导、代谢和细胞周期，表明这些分子事件在POA中的关键作用。WNT、TNFR2非经典NF-κB和刺猬信号通路以及免疫系统过程的激活在POA与ELA组中富集，这表明它们在从雌激素主导转变为孕酮主导的过程中重塑输卵管微环境时在细胞间相互作用和细胞命运决定中的作用。高度连接的上调枢纽基因ESR1、RAD51、YARS1、TYMS和CDK2可被视为在分子水平同步输卵管中POA变化的关键调节因子。
本研究揭示了与POA早期胚胎发育过程中壶腹部生理学相关的几个DEGs、信号通路和新的调节因子，这可能影响猪的繁殖力和产仔数。
