Suhaimi Suhaily, Jaafar Nardiah Rizwana, Jailani Nashriq, Ngadi Norzita, Rahman Roshanida A, Illias Rosli Md
Faculty of Chemical and Energy Engineering, Universiti Teknologi Malaysia, Skudai, Johor, 81310, Malaysia.
Appl Biochem Biotechnol. 2025 May 21. doi: 10.1007/s12010-025-05238-9.
Bioremediation using laccase (Lac) to degrade tetracycline (TC) contaminant is promising due to high specificity and selectivity of the biocatalyst. However, degradation parameters should be carefully studied to achieve maximum degradation efficiency. Thus, the current study aimed to degrade TC by employing Lac immobilized on novel carboxymethyl starch magnetic nanoparticles (CMS-MNP). The maximum TC degradation was determined via one-factor-at-a-time (OFAT) and central composite design (CCD). Using OFAT, the maximum TC degradation (56.3%) was achieved at initial TC concentration of 20 mg/mL, pH 6, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) concentration of 1.2 mM, and 10 h of reaction time. When CCD was employed, the TC degradation increased by one-fold with the highest TC degradation (62.1%) recorded at initial TC concentration of 25 mg/L, pH 5.7, ABTS concentration of 1.0 mM, and 11.8 h of reaction time. The CMS-MNP-Lac was reused for 7 cycles with a total TC degradation of 73 mg/L. The TC degradation in this study demonstrated outstanding potential and provided a green alternative for the treatment of TC contaminants in the environment.
由于生物催化剂具有高特异性和选择性，利用漆酶（Lac）进行生物修复以降解四环素（TC）污染物具有广阔前景。然而，为了实现最大降解效率，需要仔细研究降解参数。因此，本研究旨在通过使用固定在新型羧甲基淀粉磁性纳米颗粒（CMS-MNP）上的漆酶来降解TC。通过单因素实验（OFAT）和中心复合设计（CCD）确定了TC的最大降解率。使用OFAT，在初始TC浓度为20mg/mL、pH值为6、2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）浓度为1.2mM、反应时间为10小时的条件下，实现了最大TC降解率（56.3%）。当采用CCD时，TC降解率提高了一倍，在初始TC浓度为25mg/L、pH值为5.7、ABTS浓度为1.0mM、反应时间为11.8小时的条件下，记录到最高TC降解率（62.1%）。CMS-MNP-Lac可重复使用7个循环，总TC降解量为73mg/L。本研究中的TC降解显示出巨大潜力，并为环境中TC污染物的处理提供了一种绿色替代方案。
