Zheng Daiyi, Li Jiafeng, Yu Zhiyuan, Wang Ping, Li Qi, Zhao Linguo
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, China.
College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China.
Bioprocess Biosyst Eng. 2025 Aug 8. doi: 10.1007/s00449-025-03214-5.
As environmental pollution problems become increasingly severe, the treatment of persistent organic pollutants has emerged as a major challenge in the field of environmental protection. Laccase, as a green and efficient biocatalyst, demonstrates significant potential for application in environmental remediation due to its unique oxidation capabilities and broad substrate specificity. This study systematically investigated the optimization of conditions for laccase production by Coriolus versicolor, the impact of fed-batch feeding and co-cultivation with a second fungal strain on laccase secretion by C. versicolor, and the degradation performance of the produced laccase towards 2,4-dichlorophenol (2,4-DCP). The results showed that during submerged fermentation, the laccase activity of C. versicolor increased significantly over time, peaking on the 6th day, and then gradually declined due to nutrient depletion and metabolite accumulation. Optimization of wheat bran concentration (20 g/L) and initial pH value (5.0) facilitated laccase production. Additionally, fed-batch feeding during fermentation was beneficial for laccase secretion by C. versicolor. Co-cultivation with a filamentous fungus Penicillium significantly increased laccase production. On laccase-mediated degradation of 2,4-DCP, the optimal enzyme dosage (4.0 U/mL), substrate concentration (20 mg/L), and degradation time (60 h) were established. Addition of mediator 2, 2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (0.5 mmol/L) significantly improved degradation efficiency, achieving complete degradation of 2,4-DCP. HPLC analysis further verified the practical application of laccase in environmental remediation. This study provides technical support for the preparation of highly active laccase and its application in the remediation of organic pollutants through degradation.
随着环境污染问题日益严峻，持久性有机污染物的处理已成为环境保护领域的一项重大挑战。漆酶作为一种绿色高效的生物催化剂，因其独特的氧化能力和广泛的底物特异性，在环境修复中展现出巨大的应用潜力。本研究系统地考察了云芝产漆酶条件的优化、分批补料及与第二种真菌菌株共培养对云芝漆酶分泌的影响，以及所产漆酶对2,4 -二氯苯酚（2,4 - DCP）的降解性能。结果表明，在深层发酵过程中，云芝的漆酶活性随时间显著增加，在第6天达到峰值，随后由于营养物质耗尽和代谢产物积累而逐渐下降。优化麦麸浓度（20 g/L）和初始pH值（5.0）有利于漆酶的产生。此外，发酵过程中的分批补料有利于云芝分泌漆酶。与丝状真菌青霉共培养显著提高了漆酶产量。在漆酶介导的2,4 - DCP降解方面，确定了最佳酶用量（4.0 U/mL）、底物浓度（20 mg/L）和降解时间（60 h）。添加介体2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（0.5 mmol/L）显著提高了降解效率，实现了2,4 - DCP的完全降解。高效液相色谱分析进一步验证了漆酶在环境修复中的实际应用。本研究为制备高活性漆酶及其通过降解作用应用于有机污染物修复提供了技术支持。
