Johnson M P, Thompson S A, Lubaroff D M
J Urol. 1985 Jun;133(6):1112-20. doi: 10.1016/s0022-5347(17)49392-x.
Many investigators have reported effects of the pituitary hormone, prolactin, on the physiology and biochemistry of the rat prostate gland, particularly the lateral or dorsolateral lobe. The Dunning R3327H is a transplantable rat prostatic adenocarcinoma derived from a spontaneous tumor of the Copenhagen rat dorsolateral prostate. This study describes and compares morphological and physiological effects of prolactin on rat dorsolateral prostate and two sublines of the Dunning tumor. Ectopic pituitary grafts were used to induce chronic hyperprolactinemia in castrated rats receiving androgen supplement to provide a relatively controlled hormonal environment in which the effects of prolactin were maximally and consistently observed. Gravimetric and biochemical analyses, as well as ultrastructural study, provided evidence of prolactin's stimulatory effect on dorsolateral prostate growth and secretory activity. Hyperprolactinemia stimulated the growth of the well-differentiated, androgen-dependent R3327/3219 tumor subline with an increase in weight, volume and the total content of DNA, protein and zinc. There were no changes in tumor morphology. In contrast, the anaplastic androgen-independent R3327/150 tumor subline did not respond to graft-induced hyperprolactinemia. This differential response of the two R3327 tumor sublines attests to the complexity of prolactin's effects on prostatic tissue and to the extent of the deterioration of endocrine control that often accompanies tumor progression. Prolactin binding in the R3327 sublines was studied using immunohistochemical staining and radioligand assay, but produced complex results which raise questions about the discrepancy between hormone binding and biological action of prolactin in prostatic tissues.
许多研究人员报告了垂体激素催乳素对大鼠前列腺，特别是外侧叶或背外侧叶的生理和生化作用。邓宁R3327H是一种可移植的大鼠前列腺腺癌，源自哥本哈根大鼠背外侧前列腺的自发性肿瘤。本研究描述并比较了催乳素对大鼠背外侧前列腺和邓宁肿瘤两个亚系的形态学和生理学作用。异位垂体移植用于在接受雄激素补充的去势大鼠中诱导慢性高催乳素血症，以提供一个相对可控的激素环境，在其中最大程度且一致地观察催乳素的作用。重量分析、生化分析以及超微结构研究提供了证据，证明催乳素对背外侧前列腺生长和分泌活动具有刺激作用。高催乳素血症刺激了分化良好、雄激素依赖的R3327/3219肿瘤亚系的生长，其重量、体积以及DNA、蛋白质和锌的总含量均增加。肿瘤形态没有变化。相比之下，间变的雄激素非依赖型R3327/150肿瘤亚系对移植诱导的高催乳素血症没有反应。这两个R3327肿瘤亚系的不同反应证明了催乳素对前列腺组织作用的复杂性，以及肿瘤进展过程中常常伴随的内分泌控制恶化程度。使用免疫组织化学染色和放射性配体分析研究了R3327亚系中的催乳素结合情况，但结果复杂，这引发了关于前列腺组织中催乳素激素结合与生物学作用之间差异的疑问。
