Ali Aamir, Tahir Hafiz Muhammad, Ghaffar Asjid, Parveen Zahida, Munir Fariha, Muzamil Ayesha, Butt Samima Asad, Ijaz Fatima
Department of Zoology, Government College University Lahore, Lahore, Pakistan.
Pakistan Council of Scientific & Industrial Research (PCSIR), Lahore, Pakistan.
J Med Entomol. 2025 Jul 17;62(4):905-913. doi: 10.1093/jme/tjaf061.
With the increase in dengue fever cases, the control of dengue vector Aedes mosquito is crucial. The current study was designed to evaluate the larvicidal activity of essential oils of Citrus limon L.; Rutaceae and Salvia rosmarinus (Spenn 1836); Lamiaceae against Aedes aegypti L., and Aedes albopictus (Skuse 1894) mosquitoes. The essential oils were extracted by steam distillation and their component analysis was conducted. The larvicidal activities were assessed by exposing fourth instar larvae to various concentrations of essential oils for 24 h. The mode of action of essential oils was assessed by analyzing their inhibitory activities against key mosquito enzymes including acetylcholinesterase and α-amylase. The major constituents identified from both plant essential oils were 1,8-cineole, limonene, camphor, α-pinene, β-pinene, α-terpineol, and linalool. Moreover, the C. limon and S. rosmarinus essential oils exhibited significant larvicidal activities against Aedes larvae. The LC50 values of C. limon essential oil for Ae. aegypti and Ae. albopictus larvae were 33.43 and 38.01 mg/liter, respectively, as compared to the LC50 of S. rosmarinus essential oil that were 44.96 and 49.53 mg/liter, respectively. Furthermore, both plant essential oils significantly inhibited the activities of acetylcholinesterase and α-amylase. The camphor and limonene were the major essential oils components with highest binding affinities against acetylcholinesterase (-6.3 and -6.4) and α-amylase (-5.9 and -5.2) enzymes. Therefore, it can be concluded that the essential oils of C. limon and S. rosmarinus with diverse compositions possess significant larvicidal activities as well as inhibitory activities against acetylcholinesterase and α-amylase. Therefore, these essential oils can be used for Aedes mosquito control.
随着登革热病例的增加，控制登革热传播媒介伊蚊至关重要。当前的研究旨在评估芸香科植物柠檬（Citrus limon L.）和唇形科植物迷迭香（Salvia rosmarinus (Spenn 1836)）的精油对埃及伊蚊（Aedes aegypti L.）和白纹伊蚊（Aedes albopictus (Skuse 1894)）的杀幼虫活性。通过水蒸气蒸馏法提取精油并进行成分分析。通过将四龄幼虫暴露于不同浓度的精油中24小时来评估杀幼虫活性。通过分析精油对包括乙酰胆碱酯酶和α -淀粉酶在内的关键蚊虫酶的抑制活性来评估其作用方式。从这两种植物精油中鉴定出的主要成分是1,8 -桉叶素、柠檬烯、樟脑、α -蒎烯、β -蒎烯、α -松油醇和芳樟醇。此外，柠檬和迷迭香的精油对伊蚊幼虫表现出显著的杀幼虫活性。柠檬精油对埃及伊蚊和白纹伊蚊幼虫的半数致死浓度（LC50）分别为33.43和38.01毫克/升，相比之下，迷迭香精油的LC50分别为44.96和49.53毫克/升。此外，两种植物精油均显著抑制乙酰胆碱酯酶和α -淀粉酶的活性。樟脑和柠檬烯是对乙酰胆碱酯酶（结合亲和力分别为 -6.3和 -6.4）和α -淀粉酶（结合亲和力分别为 -5.9和 -5.2）具有最高结合亲和力的主要精油成分。因此，可以得出结论，成分各异的柠檬和迷迭香精油具有显著的杀幼虫活性以及对乙酰胆碱酯酶和α -淀粉酶的抑制活性。因此，这些精油可用于控制伊蚊。
