Cheng Sen-Sung, Chang Hui-Ting, Lin Chun-Ya, Chen Pin-Sheng, Huang Chin-Gi, Chen Wei-June, Chang Shang-Tzen
Experimental Forest, National Taiwan University, Nan-Tou 55750, Taiwan.
Pest Manag Sci. 2009 Mar;65(3):339-43. doi: 10.1002/ps.1693.
The current study investigates, for the first time, the mosquito larvicidal activities of leaf and twig essential oils from Clausena excavata Burm. f. and their individual constituents against Aedes aegypti L. and Aedes albopictus Skuse larvae. The yields of essential oils obtained from hydrodistillation were compared, and their constituents were determined by GC-MS analyses.
The LC(50) values of leaf and twig essential oils against fourth-instar larvae of Ae. aegypti and Ae. albopictus were 37.1-40.1 microg mL(-1) and 41.1-41.2 microg mL(-1) respectively. This study demonstrated that C. excavata leaf and twig essential oils possess mosquito larvicidal activity, inhibiting the growth of mosquito larvae for both species at a low concentration. In addition, results of larvicidal assays showed that the effective constituents in leaf and twig essential oils were limonene, gamma-terpinene, terpinolene, beta-myrcene, 3-carene and p-cymene. The LC(50) values of these constituents against both mosquito larvae were below 50 microg mL(-1). Among these effective constituents, limonene had the best mosquito larvicidal activity, with LC(50) of 19.4 microg mL(-1) and 15.0 microg mL(-1) against Ae. aegypti and Ae. albopictus larvae respectively.
The findings suggested that the essential oils from Clausena excavata leaf and twig and their effective constituents may be explored as a potential natural larvicide.
本研究首次调查了黄皮叶和嫩枝精油及其各成分对埃及伊蚊和白纹伊蚊幼虫的杀蚊活性。比较了水蒸气蒸馏法获得的精油产量，并通过气相色谱 - 质谱分析确定了其成分。
黄皮叶和嫩枝精油对埃及伊蚊和白纹伊蚊四龄幼虫的LC(50)值分别为37.1 - 40.1微克/毫升和41.1 - 41.2微克/毫升。本研究表明，黄皮叶和嫩枝精油具有杀蚊幼虫活性，能在低浓度下抑制两种蚊子幼虫的生长。此外，杀幼虫试验结果表明，叶和嫩枝精油中的有效成分是柠檬烯、γ-萜品烯、萜品油烯、β-月桂烯、3-蒈烯和对伞花烃。这些成分对两种蚊子幼虫的LC(50)值均低于50微克/毫升。在这些有效成分中，柠檬烯的杀蚊幼虫活性最佳，对埃及伊蚊和白纹伊蚊幼虫的LC(50)分别为19.4微克/毫升和15.0微克/毫升。
研究结果表明，黄皮叶和嫩枝精油及其有效成分有望开发成为一种潜在的天然杀幼虫剂。
