Xu Yanwei, Xiao Yong, Ai Chunxiao, Huang Yihong, Zhou Huajin, Li Jianhui, Yuan Jianmin
State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China; College of Animal Science, Shanxi Agricultural University, Taigu, 030801, China.
State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Poult Sci. 2025 May 10;104(8):105288. doi: 10.1016/j.psj.2025.105288.
This study aimed to investigate the internal connections between starch digestion properties and microbial changes in the gastrointestinal tract of broiler chickens. A total of 240 male AA+ broilers (21-day-old) were allocated to 4 treatments of regular and waxy corn with and without virginiamycin (6.4 g/t) in a 2 × 2 factorial arrangement. On day 35, growth performance and apparent starch digestibility were determined, and hormones in the serum and microbial profiles in the crop and ileum were analyzed. The results indicated that dietary starch was primarily digested in the jejunum of broiler chickens, and broilers receiving waxy corn exhibited significantly greater digestibility of both total starch and amylopectin in the distal jejunum, along with an improved feed-to-gain ratio (F/G) and European performance index (EPI), compared with the regular corn group (P< 0.05). Notably, virginiamycin significantly enhanced the amylose digestibility in the distal ileum and improved the F/G ratio (P< 0.05). Microbial analyses revealed that corn type did not affect microbial diversity in the crop and ileum. However, virginiamycin significantly reduced alpha diversity in the crop (Chao1: 129.63 vs. 191.14, P < 0.05) and ileum (observed species: 137.23 vs. 316.43, P < 0.05), and elevated Lactobacillus abundance (positively correlated with amylose digestibility, P < 0.001). In summary, waxy corn enhanced jejunal starch hydrolysis via its high amylopectin content, whereas virginiamycin promoted amylose digestion in the distal small intestine by selectively enriching the starch-degrading microbiota.
本研究旨在探究淀粉消化特性与肉鸡胃肠道微生物变化之间的内在联系。总共240只雄性AA +肉鸡（21日龄）按照2×2析因设计被分配到4种处理组，分别为添加和不添加维吉尼亚霉素（6.4 g/t）的普通玉米和糯玉米。在第35天，测定生长性能和表观淀粉消化率，并分析血清中的激素以及嗉囊和回肠中的微生物图谱。结果表明，日粮淀粉主要在肉鸡空肠中消化，与普通玉米组相比，饲喂糯玉米的肉鸡在空肠远端的总淀粉和支链淀粉消化率显著更高，饲料转化率（F/G）和欧洲生产性能指数（EPI）也有所改善（P<0.05）。值得注意的是，维吉尼亚霉素显著提高了回肠远端的直链淀粉消化率并改善了F/G比值（P<0.05）。微生物分析表明，玉米类型不影响嗉囊和回肠中的微生物多样性。然而，维吉尼亚霉素显著降低了嗉囊（Chao1指数：129.63对191.14，P < 0.05）和回肠（观测物种数：137.23对316.43，P < 0.05）中的α多样性，并提高了乳酸杆菌丰度（与直链淀粉消化率呈正相关，P < 0.001）。综上所述，糯玉米通过其高支链淀粉含量增强了空肠淀粉水解，而维吉尼亚霉素通过选择性富集降解淀粉的微生物群促进了小肠远端的直链淀粉消化。
