一对人类嵌合双胞胎的XX和XY细胞中不同的姐妹染色单体交换率。

Different sister chromatid exchange rates in XX and XY cells of a pair of human chimeric twins.

作者信息

Wulf H C, Niebuhr E

出版信息

Cytogenet Cell Genet. 1985;39(2):105-8. doi: 10.1159/000132116.

Abstract

To explore the influence of sex on sister chromatid exchange (SCE) level, a pair of chimeric twins was examined for differences in SCE frequency between the XX and XY cells present in each individual. By this method, the influence of possible differences in environmental exposure was eliminated. SCE levels were varied by growing the cells in media containing 0, 1.3 X 10(-7), or 6.5 X 10(-7) M melphalan. XX cells showed a higher SCE count than XY cells. This difference increased with increasing SCE level and ranged from 5.4% to 7.8% (P = 0.0003) of the SCE counts. Only about 2% of the difference could be explained by the higher amount of DNA present in the XX cells than in the XY cells. In this case XX cells seemed to be more sensitive to SCE-inducing agents than XY cells.

摘要

为了探究性别对姐妹染色单体交换（SCE）水平的影响，对一对嵌合双胞胎进行了检查，以分析每个个体中XX细胞和XY细胞之间SCE频率的差异。通过这种方法，消除了环境暴露可能存在的差异的影响。通过在含有0、1.3×10⁻⁷或6.5×10⁻⁷ M美法仑的培养基中培养细胞，使SCE水平发生变化。XX细胞的SCE计数高于XY细胞。这种差异随着SCE水平的增加而增大，范围为SCE计数的5.4%至7.8%（P = 0.0003）。差异中只有约2%可归因于XX细胞中存在的DNA量高于XY细胞。在这种情况下，XX细胞似乎比XY细胞对SCE诱导剂更敏感。