Combination of Quercetin and Curcumin modulates NF-κB inflammatory signaling pathway in murine model of esophageal erosive reflux disease erosive reflux disease.

BACKGROUND

This study aimed to investigate whether quercetin, in combination with curcumin, modulates the nuclear factor-kappa B (NF-κB) inflammatory signaling pathway in a murine model of esophageal erosive reflux disease.

MATERIALS AND METHODS

The expression levels of NF-κB and inflammatory factors such as interleukin-1beta (IL-1β), IL-8, and tumor necrosis factor-alpha (TNF-α) were analyzed using Real-Time PCR. Additionally, enzyme-linked immunosorbent assay (ELISA) was employed to validate the Real-Time PCR findings. The western blot study was also conducted to determine the effects on NF-kB p-p65, TNF-α, IL-1β, IL-8.

RESULTS

The results demonstrated that the combination of quercetin and curcumin significantly suppressed the NF-κB inflammatory pathway by reducing the expression of various examined pro-inflammatory cytokines in the murine model of esophageal erosive reflux disease, achieving greater efficacy than when either compound was used individually.

CONCLUSIONS

The findings suggest that the synergistic effect of quercetin and curcumin offers a promising approach to mitigating inflammation via modulation of the NF-κB pathway in esophageal erosive reflux disease.