Shopsis C, Borenfreund E, Walberg J, Stark D M
Food Chem Toxicol. 1985 Feb;23(2):259-66. doi: 10.1016/0278-6915(85)90026-2.
Four assays that may serve as components of a battery of alternatives to the conventional Draize test were described. The exfoliative cytology assay is a refinement of the Draize test that may provide a more sensitive and more objective end-point. The macrophage migration assay addresses the inflammatory aspects of the physiological response to irritation. The uridine uptake inhibition assay uses a quantitative, reversible end-point to detect the short-term action of agents on cell membranes and cell phosphorylative potential. Finally, the cytological assay serves as a rapid, easily performed general indicator of cytotoxic action. The two latter assays have been demonstrated to correlate very well with Draize test results and with each other for a wide range of test agents.
描述了四种可作为传统兔眼刺激试验替代方法组合组成部分的试验。脱落细胞学试验是对兔眼刺激试验的改进，可能会提供更敏感、更客观的终点。巨噬细胞迁移试验关注对刺激的生理反应中的炎症方面。尿苷摄取抑制试验使用定量、可逆的终点来检测试剂对细胞膜和细胞磷酸化潜能的短期作用。最后，细胞学试验可作为细胞毒性作用的快速、易于执行的一般指标。已证明后两种试验与兔眼刺激试验结果以及在广泛的受试试剂中彼此之间具有很好的相关性。
