Song Xue, Li Haotian, Fu Chuhan, Li Jiahong, Xiang Jihong, Sun Xuetong, Liu Jielin, Qin Ligang
Department of Grassland Science, College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
Grassland Station of Heilongjiang, Harbin, China.
Front Plant Sci. 2025 May 7;16:1571736. doi: 10.3389/fpls.2025.1571736. eCollection 2025.
Drought stress severely impacts plant productivity, particularly in non-cultivated species such as .L. However, the role of rhizosphere and endophytic bacterial communities in enhancing drought tolerance remains underexplored.
We used 16S rRNA amplicon sequencing to investigate microbial communities in the rhizosphere, roots, bulbs, and leaves of under simulated drought conditions using PEG-6000 (CK, 5%, 15%, 25%) and post-rehydration recovery. Alpha and beta diversity, phylogenetic relationships, and functional predictions were analyzed.
Drought stress reduced rhizosphere bacterial diversity by 42% but increased leaf diversity by 52%. The 15% PEG treatment marked a key threshold for community shifts. and were significantly enriched under drought, and functional predictions indicated their involvement in osmotic regulation and phytohormone synthesis. Post-rehydration partially restored microbial composition in aerial tissues but not in the rhizosphere.
These findings suggest that drought induces niche-specific microbial adaptations and that bacterial community structure plays a critical role in drought resilience. This study provides insights into plant-microbe interactions and offers a basis for developing microbial strategies to improve drought tolerance in species.
干旱胁迫严重影响植物生产力，尤其是对于像[具体物种名称]这样的非栽培物种。然而，根际和内生细菌群落在增强耐旱性方面的作用仍未得到充分探索。
我们使用16S rRNA扩增子测序来研究在使用聚乙二醇6000（对照、5%、15%、25%）模拟干旱条件下及复水恢复后[具体物种名称]的根际、根、鳞茎和叶片中的微生物群落。分析了α和β多样性、系统发育关系以及功能预测。
干旱胁迫使根际细菌多样性降低了42%，但使叶片多样性增加了52%。15%的聚乙二醇处理标志着群落转变的关键阈值。[具体细菌种类1]和[具体细菌种类2]在干旱条件下显著富集，功能预测表明它们参与渗透调节和植物激素合成。复水后地上组织中的微生物组成部分恢复，但根际未恢复。
这些发现表明干旱诱导了特定生态位的微生物适应性，并且细菌群落结构在干旱恢复力中起着关键作用。本研究为植物 - 微生物相互作用提供了见解，并为开发提高[具体物种名称]物种耐旱性的微生物策略提供了基础。
