Competitive physical sports demand rigorous training, increasing the risk of overuse-associated musculoskeletal traumatic injuries followed by a complex and time-consuming healing process with economic effects and potential disability. Tissue healing involves inflammation, molecular and cellular pathway regulation, proliferation, and tissue regeneration. These responses can significantly vary depending on the location and severity of the injury, affecting recovery time, pain intensity, range of motion, and return to sports activity. Despite medical advancements, healing, pain alleviation, regenerative tissue quality, mobility, and quality of life remain challenging. Current treatments, including nonsteroidal anti-inflammatory drugs and opioid-based treatments, have systemic adverse effects and efficacy limitations. Long-duration ultrasound therapy has emerged as a promising mechanobiological and diathermic treatment, providing critical biomechanical and thermal stimuli. Biomechanical stimuli help regulate acute inflammation, cellular proliferation, and tissue regeneration. Thermal stimuli enhance blood flow, angiogenesis, and nutrient exchange, accelerating healing and improving recovery outcomes. These combined stimuli also increase skin porosity and permeability, facilitating targeted drug delivery through sonoporation and enhancing the efficacy of treatments like platelet-rich plasma therapy. This review examines recent studies exploring the therapeutic effects of long-duration ultrasound as a standalone and adjunctive therapy. It examined its roles in regulating acute inflammation, mitigating chronic inflammation, tissue regeneration, healing, sports-associated pain management, mobility, and overall tissue recovery.