Edel B, Brückner B, Hänel I, Tröger R
J Basic Microbiol. 1985;25(3):155-60. doi: 10.1002/jobm.3620250304.
The synthesis of a phytotoxic substance, tentoxin, by Alternaria alternata (Fr.) Keissler is negatively controlled by inorganic phosphate. We show here that the influence of phosphate on the production of this secondary metabolite is time-dependent. By using inhibitors of protein synthesis (p-fluorphenylalanine, cycloheximide) together with a resuspension technique, attempts were made to find out when the enzymes responsible for tentoxin synthesis are formed.
链格孢菌(Alternaria alternata (Fr.) Keissler)合成的植物毒性物质——细交链孢菌酮酸,受到无机磷酸盐的负调控。我们在此表明,磷酸盐对这种次生代谢产物产生的影响具有时间依赖性。通过使用蛋白质合成抑制剂(对氟苯丙氨酸、环己酰亚胺)并结合重悬技术,试图找出负责细交链孢菌酮酸合成的酶是何时形成的。