An Soyeong, Kim Youngseo, Lee Ja Yil
Department of Biological Sciences, Ulsan National Institute of Science and Technology, Ulsan 44919, Republic of Korea.
Department of Biological Sciences, Ulsan National Institute of Science and Technology, Ulsan 44919, Republic of Korea; Institute of Basic Science Center for Genomic Integrity, Ulsan 44919, Republic of Korea.
Mol Cells. 2025 Jul;48(7):100224. doi: 10.1016/j.mocell.2025.100224. Epub 2025 May 20.
The advancement of single-molecule imaging techniques has significantly enhanced our understanding of biomolecular reactions and cellular processes that remain obscured in ensemble measurements. In particular, DNA curtains are high-throughput hybrid methods integrating total internal reflection fluorescence microscopy, lipid fluidity, microfluidics, and nano-fabrication, enabling the direct visualization of protein-DNA interactions in real time. The techniques have emerged as powerful tools for probing molecular dynamics of diverse DNA metabolic processes, including DNA damage repair and chromatin dynamics. This review not only highlights recent applications of DNA curtain techniques for elucidating mechanisms underlying DNA damage repair and chromatin dynamics, but also shows how DNA curtain techniques have provided novel insights into the interplay between DNA metabolic processes in the chromatin context.
单分子成像技术的进步显著增进了我们对生物分子反应和细胞过程的理解，而这些在整体测量中仍难以看清。特别是，DNA帘幕是一种高通量杂交方法，整合了全内反射荧光显微镜、脂质流动性、微流体技术和纳米制造技术，能够实时直接观察蛋白质与DNA的相互作用。这些技术已成为探究各种DNA代谢过程分子动力学的有力工具，包括DNA损伤修复和染色质动力学。本综述不仅强调了DNA帘幕技术在阐明DNA损伤修复和染色质动力学机制方面的最新应用，还展示了DNA帘幕技术如何为染色质环境中DNA代谢过程之间的相互作用提供了新的见解。
