Javadov Toghrul, Unal Semra, Katmis Asli, Sakar Mustafa, Akakin Dilek, Demirci Elif Kervancioglu, Ziyal Mustafa Ibrahim
From the Department of Neurosurgery, School of Medicine, Institute of Neurological Sciences, Marmara University, Istanbul, Turkey.
Institute of Neurological Sciences, Marmara University, Istanbul, Turkey.
ASAIO J. 2025 Sep 1;71(9):775-783. doi: 10.1097/MAT.0000000000002463. Epub 2025 May 23.
The aim of this study was to investigate the treatment effect of an artificial nerve graft produced with polylactic acid (PLA) and polyvinylpyrrolidone (PVP) by electrospinning method, in combination with rat allogeneic adipose tissue-derived mesenchymal stem cells (rAT-MSCs), on axonal regeneration in a rat sciatic nerve injury. A 10 mm long gap in the right sciatic nerve was created in rats which were divided into three groups and the gap was repaired with autologous nerve graft (autograft group), PLA/PVP conduit alone (PLA/PVP group), and PLA/PVP conduit with rAT-MSCs inside (PLA/PVP with rAT-MSC group). At the end of the eighth week, the results were evaluated functionally, electrophysiologically, and histologically. In functional evaluation, sciatic functional index (SFI) was used and all groups showed improvement over time. The results were seen in the autograft and PLA/PVP with rAT-MSC groups, with no statistically significant difference between them. In electrophysiological evaluation, action potential was seen in all three groups, with the best results seen in the PLA/PVP with rAT-MSC group with statistical significance. Histological evaluation showed axonal regeneration in all three groups. These results show that PLA/PVP conduit contributes to regeneration even when used alone, and increases regeneration when used together with rAT-MSCs.
本研究旨在探讨通过静电纺丝法制备的聚乳酸（PLA）和聚乙烯吡咯烷酮（PVP）与大鼠同种异体脂肪组织来源的间充质干细胞（rAT-MSCs）相结合的人工神经移植物对大鼠坐骨神经损伤后轴突再生的治疗效果。在大鼠右侧坐骨神经上制造一个10毫米长的间隙，将大鼠分为三组，分别用自体神经移植物（自体移植组）、单独的PLA/PVP导管（PLA/PVP组）以及内部含有rAT-MSCs的PLA/PVP导管（含rAT-MSCs的PLA/PVP组）修复该间隙。在第八周结束时，从功能、电生理和组织学方面对结果进行评估。在功能评估中，使用坐骨神经功能指数（SFI），所有组均随时间有所改善。自体移植组和含rAT-MSCs的PLA/PVP组均有效果，二者之间无统计学显著差异。在电生理评估中，三组均出现动作电位，含rAT-MSCs的PLA/PVP组效果最佳，具有统计学意义。组织学评估显示三组均有轴突再生。这些结果表明，PLA/PVP导管即使单独使用也有助于再生，与rAT-MSCs一起使用时可增强再生效果。
