Sun Xinhang, Bundock Peter, Mason Patrick, Poudel Pragya Dhakal, Varshney Rajeev, Topp Bruce, Alam Mobashwer
Queensland Alliance for Agriculture & Food Innovation, The University of Queensland, Nambour, QLD 4560, Australia.
Southern Cross Plant Science, Southern Cross University, Lismore, NSW 2480, Australia.
BioTech (Basel). 2025 May 16;14(2):37. doi: 10.3390/biotech14020037.
Evaluating the genetic variability of germplasms is essential for enhancing and developing superior cultivars. However, there is limited information on cultivated germplasm diversity for Australian passion fruit breeding programs. The genetic diversity of Australian passion fruit ( spp.), including 94 rootstocks and 95 scions, was evaluated to support breeding programs aimed at enhancing productivity, fruit quality, and overall crop resilience. Rootstocks were genotyped using high-density 24k Diversity Arrays Technology (DArT)-based single-nucleotide polymorphism (SNP) markers, while genetic characterization of scions was conducted using eight simple sequence repeat (SSR) markers. The resulting genetic relationships revealed significant variation within rootstock populations. Bayesian cluster analysis in STRUCTURE showed that the rootstock population was divided into six distinct genetic groups, whereas only two subpopulations were identified among the scion accessions. SNP-based genotyping further highlighted the allelic diversity of Australian rootstocks, suggesting a rich reservoir of genetic traits for rootstock improvement. These findings underscore the importance of preserving and utilizing genetic diversity in Australian passion fruit germplasm to drive the development of superior cultivars with enhanced adaptability and performance under diverse environmental conditions.
评估种质的遗传变异性对于改良和培育优良品种至关重要。然而，关于澳大利亚百香果育种计划中栽培种质多样性的信息有限。对包括94种种源和95个接穗的澳大利亚百香果（西番莲属）的遗传多样性进行了评估，以支持旨在提高生产力、果实品质和整体作物抗逆性的育种计划。使用基于高密度24k多样性阵列技术（DArT）的单核苷酸多态性（SNP）标记对种源进行基因分型，而使用八个简单序列重复（SSR）标记对接穗进行遗传特征分析。由此产生的遗传关系揭示了种源群体内的显著变异。STRUCTURE中的贝叶斯聚类分析表明，种源群体分为六个不同的遗传组，而在接穗种质中仅鉴定出两个亚群。基于SNP的基因分型进一步突出了澳大利亚种源的等位基因多样性，表明存在丰富的遗传性状库可用于种源改良。这些发现强调了保护和利用澳大利亚百香果种质遗传多样性以推动培育在不同环境条件下具有更强适应性和性能的优良品种的重要性。
