一种从耐辐射球菌中分离出的Z-DNA结合蛋白。

A Z-DNA binding protein isolated from D. radiodurans.

作者信息

Kitayama S, Matsumura O, Masuda S

出版信息

Biochem Biophys Res Commun. 1985 Aug 15;130(3):1294-300. doi: 10.1016/0006-291x(85)91755-3.

Abstract

A DNA binding protein isolated from D. radiodurans changes CD-spectrum of Z-form poly(dG-dC) X poly(dG-dC). We have found that a positive band at 268 nm is converted close to that of B-form in the presence of the protein. Concomitantly, a negative band at 295 nm shown by Z-form poly(dG-dC) X poly (dG-dC) was weakened by the protein but not by albumin. Such changes in the CD-spectra were not induced by the protein and by albumin when they were mixed with Z- or B-form poly(dG-me5dC) X poly(dG-me5dC) or with B-form poly(dG-dC) X poly(dG-dC). The protein formed a complex preferentially with Z-form poly(dG-dC) X poly(dG-dC).

摘要

从耐辐射球菌中分离出的一种DNA结合蛋白可改变Z型聚（dG-dC）×聚（dG-dC）的圆二色光谱。我们发现，在该蛋白存在的情况下，268nm处的正峰转变为接近B型的峰。与此同时，Z型聚（dG-dC）×聚（dG-dC）在295nm处的负峰被该蛋白减弱，但白蛋白不会使其减弱。当该蛋白和白蛋白与Z型或B型聚（dG-me5dC）×聚（dG-me5dC）或B型聚（dG-dC）×聚（dG-dC）混合时，不会诱导圆二色光谱发生此类变化。该蛋白优先与Z型聚（dG-dC）×聚（dG-dC）形成复合物。